Light measurement device, light measurement method, and light measurement program

A light measurement method for measuring light from a sample including a myocardial cell, comprising:

• acquiring luminance-value data from a pixel of an image of the sample over time, wherein the image of the sample comprises an image of the myocardial cell, the pixel is associated with a location within the sample, and the acquired luminance-value data indicates a cyclical temporal alteration in light detected at the location of the sample,
• determining a presence of the myocardial cell at the location of the sample by detecting cycles of the luminance-value data corresponding to a pulsing of the myocardial cell, based on a comparison of the luminance-value data with at least one threshold associated with the pulsing of the myocardial cell to obtain the cyclical temporal alteration,
• determining the cyclical temporal alteration by extracting a plurality of peak values of the luminance value from the luminance-value data,
• calculating at least one evaluation value for each of the cycles of the luminance-value data,
• calculating the number of times the peak value exceeds the threshold by comparing the peak value with the threshold value,
• determining an average value from the at least one evaluation value calculated for each of the cycles of the luminance-value data,
• determining the presence of the myocardial cell at the location of the sample by comparing the average value with the at least one threshold, and
• identifying a target pixel based on the number of times the peak value exceeds the threshold value, the target pixel forming at least a part of the image of the myocardial cell.

A non-transitory computer readable medium having stored thereon one or more sequences of instructions for causing one or more processors to perform the steps for measuring light from a sample including a myocardial cell, the steps comprising:

• acquiring luminance-value data from a pixel of an image of the sample over time, wherein the image of the sample comprises an image of the myocardial cell, the pixel is associated with a location within the sample, and the acquired luminance-value data indicates a cyclical temporal alteration in light detected on the location of the sample,
• evaluating the determining a presence of the myocardial cell at the location of the sample by detecting cycles of the luminance-value data corresponding to a pulsing of the myocardial cell, based on a comparison of the luminance-value data with at least one threshold associated with the pulsing of the myocardial cell to obtain the cyclical temporal alteration,
• identifying the pixel associated with the luminance-value data as a target pixel that forms at least part of the image of the myocardial cell,
• determining the cyclical temporal alteration by extracting a plurality of peak values of the luminance value from the luminance-value data,
• calculating at least one evaluation value for each of the cycles of the luminance-value data,
• calculating the number of times the peak value exceeds the threshold by comparing the peak value with the threshold value, and
• determining an average value from the at least one evaluation value calculated for each of the cycles of the luminance-value data,
• determining the presence of the myocardial cell at the location of the sample by comparing the average value with the at least one threshold, and
• identifying the target pixel based on the number of times the peak value exceeds the threshold value.