Methods for normalizing background effects in multiplex immunoassays
AssigneeThe United States of America as represented by the Secretary of the Army on behalf of the U.S. Army Medical Research and Materiel Command
InventorsNeal G. Satterly, Randal J. Schoepp
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Abstract
Disclosed herein methods, compositions, and kits for obtaining a normalized measured amount of a target in a sample by measuring the amount of the target in the sample by contacting the sample with a detection reagent, measuring the amount of a ligand known to be absent in the sample being tested by contacting the sample with a control reagent, and obtaining the normalized measured amount of the target by calculating the ratio of the measured amount of the target to the measured amount of the ligand.
Claims16
A method for obtaining a normalized measured amount of a given target in a sample using a multiplex immunoassay which comprises
- measuring the amount of the given target in the sample by contacting the sample with a detection reagent for the given target,
- measuring the amount of an M13 bacteriophage protein in the sample using an antibody specific for the M13 bacteriophage protein, and
- obtaining the normalized measured amount of the given target by calculating the ratio of the measured amount of the given target to the measured amount of the M13 bacteriophage protein.
A method for identifying a sample as containing or not containing a given target which comprises
- using a multiplex immunoassay to measure the amount of the given target in the sample by contacting the sample with a detection reagent for the given target and measure the amount of an M13 bacteriophage protein in the sample by contacting the sample with an antibody specific for the M13 bacteriophage protein,
- obtaining the normalized measured amount of the given target by calculating the ratio of the measured amount of the given target to the measured amount of the M13 bacteriophage protein, and
- identifying the sample as containing the given target where the normalized measured amount of the given target is a positive number.
Dependencies are inferred from the claim text.