Calibration reagent and method
AssigneePhadia AB
InventorsGöran Elfverson, Per Matsson, Mats Nystrand
Life of this patent
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Abstract
The present invention relates to a method for calibrating a multiplex assay, comprising: adding a calibration reagent to a solid phase on which a plurality of capturing agents are immobilized, adding a detection molecule which has a capacity to bind to the calibration reagent, detecting bound detection molecule, thereby creating a calibration curve, wherein the calibration reagent comprises at least two different binding molecules, wherein each binding molecule has a capacity to bind specifically to a capturing agent immobilized on the solid phase and a capacity to bind to a detection molecule. Further provided is a multiplex assay system comprising such a calibration reagent.
Claims24
A method for calibrating a multiplex assay operable to detect a plurality of different analytes in a sample, comprising:
- providing a solid phase with a plurality of capturing agents for the plurality of different analytes, respectively, immobilised on the solid phase,
- adding a calibration reagent to the solid phase on which the plurality of capturing agents are immobilised, wherein the calibration reagent comprises at least two different binding molecules, wherein each binding molecule has a capacity to bind specifically to one of the plurality of capturing agents immobilised on the solid phase, wherein at least two of the binding molecules have different specificities, and wherein of said at least two binding molecules, a first binding molecule is present in a first concentration and a second binding molecule is present in a second concentration, different from the first concentration,
- adding a first detection molecule to the solid phase, wherein each binding molecule has a capacity to bind to the first detection molecule,
- detecting the bound first detection molecule, and
- creating a single calibration curve comprising a plurality of calibration points, wherein the plurality of calibration points include at least two points, wherein a first of the points is from detection of the first detection molecule bound to the first binding molecule only, present in the first concentration, and wherein a second of the points is from detection of the first detection molecule bound to the second binding molecule only, present in the second concentration, respectively.
A method for calibrating a multiplex assay operable to individually quantitatively detect a plurality of different analytes in a sample, comprising:
- providing a solid phase with a plurality of capturing agents for the plurality of different analytes, respectively, immobilised on the solid phase,
- adding a calibration reagent to the solid phase on which the plurality of capturing agents are immobilised, wherein the calibration reagent comprises at least four different binding molecules, wherein each binding molecule has a capacity to bind specifically to one of the plurality of capturing agents immobilised on the solid phase, wherein at least four of the binding molecules have different specificities, wherein of said at least four binding molecules, a first binding molecule is present in a first concentration, a second binding molecule is present in a second concentration, a third binding molecule is present in a third concentration, and a fourth binding molecule is present in a fourth concentration, and wherein the first, second, third and fourth concentrations differ from one another,
- adding a first detection molecule to the solid phase, wherein each binding molecule has a capacity to bind to the first detection molecule,
- detecting the bound first detection molecule, and
- creating a single calibration curve comprising a plurality of calibration points, wherein the plurality of calibration points include at least four points, wherein a first of the points is from detection of the first detection molecule bound to the first binding molecule only, present in the first concentration, a second of the points is from detection of the first detection molecule bound to the second binding molecule only, present in the second concentration, a third of the points is from detection of the first detection molecule bound to the third binding molecule only, present in the third concentration, and a fourth of the points is from detection of the first detection molecule bound to the fourth binding molecule only, present in the fourth concentration, respectively.
Dependencies are inferred from the claim text.