Antibodies to aripiprazole haptens and use thereof
AssigneeJanssen Pharmaceutica NV
InventorsEric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M. Remmerie, Rhys Salter, Ronghui Lin
Life of this patent
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Abstract
Disclosed is an antibody which binds to aripiprazole, which can be used to detect aripiprazole in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of aripiprazole, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
Claims8
A method of producing an antibody which binds to aripiprazole, the method comprising:
- (i) selecting a host for antibody production;
- and
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier, wherein the host produces an antibody which binds to aripiprazole,
- wherein:
- R1 is H,
- CH2NH2, or
- CH2NHC(O)(CH2)mCO2H;
- R2 is H,
- NH2, or NHC(O)(CH2)mCO2H;
- R3 is H;
- provided that either R1 or R2 must be H, and further provided that R1 and R2 may not all be H simultaneously;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5.
A method of producing a hybridoma cell line capable of producing a monoclonal antibody which binds to aripiprazole, the method comprising:
- (i) selecting a host for antibody production;
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier;
- (iii) fusing a cell line from said inoculated host with a continuously dividing cell to create a fused cell capable of producing a monoclonal antibody which binds to aripiprazole;
- and
- (iv) cloning the fused cell so as to obtain a hybridoma cell line,
- wherein:
- R1 is H,
- CH2NH2 or
- CH2NHC(O)(CH2)mCO2H;
- R2 is H,
- NH2, or NHC(O)(CH2)mCO2H;
- R3 is H;
- provided that either R1 or R2 must be H, and further provided that R1 and R2 may not all be H simultaneously;
- m is 1, 2, 3, 4, or 5;
- and
- n is 1, 2, 3, 4, or 5.
Dependencies are inferred from the claim text.