Charge reversible ion exchange resins, chromatography column, method, and system thereof
AssigneeDionex Corporation
InventorChristopher A. Pohl
Life of this patent
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Abstract
An ion exchange chromatographic packing material is described that includes a copolymer grafted to support resin particles. The copolymer includes an ion exchange group, an ionic crosslinking group configured to ionically bind to the ion exchange group, and an adjustable ionization state group having at least a first net charge at the first pH and a second net charge at the second pH. An overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to the overall second net charge of the chromatographic packing material. This allows impurities to be removed from the chromatographic packing material at the second pH.
Claims38
An ion exchange chromatographic packing material comprising:
- a) a support resin particle;
- and
- b) a copolymer grafted to the support resin particle, the copolymer comprising:
- i) an ion exchange group configured to have a polarity that does not change when switching from use with a first eluent having a first pH to use with a second eluent having a second pH, in which the first pH and the second pH different,
- ii) an ionic crosslinking group configured to ionically bind to the ion exchange group,
- iii) an adjustable ionization state group configured to have a first net charge at the first pH and a second net charge at the second pH, in which the first net charge and the second net charge different,
- wherein an overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to an overall second net charge of the chromatographic packing material at the second pH.
An ion exchange chromatographic packing material made by a method comprising:
- combining a support resin particle, an ion exchange monomer, an ionic crosslinking monomer, and an adjustable ionization state monomer to form a reaction mixture;
- forming a copolymer of the ion exchange monomer, the ionic crosslinking monomer, and the adjustable ionization state monomer;
- and
- grafting the copolymer to the support resin particle,
- in which the ion exchange monomer, the ionic crosslinking monomer, and the adjustable ionization state monomer are selected such that the copolymer comprises:
- i) a corresponding ion exchange group configured to have a polarity that does not change when switching from use with a first eluent having a first pH to use with a second eluent having a second pH, in which the first pH and the second pH different,
- ii) a corresponding ionic crosslinking group configured to ionically bind to the ion exchange group,
- iii) a corresponding adjustable ionization state group configured to have a first net charge at the first pH and a second net charge at the second pH, in which the first net charge and the second net charge different,
- wherein an overall first net charge of the chromatographic packing material at the first pH is opposite in polarity to an overall second net charge of the chromatographic packing material at the second pH, and.
Dependencies are inferred from the claim text.