Sample collection device and manufacturing method thereof

A sample collection device, comprising:

• two substrates, disposed oppositely to each other, wherein each of the substrates has a first surface, a second surface opposite to the first surface, a first recess and at least one second recess, the two substrates are disposed with the first surfaces facing each other, the first recess and the at least one second recess are respectively sunk into the first surface and collectively channeled through the each of the substrates, the first recess and the at least one second recess are spatially interconnected and fluidly communicated with each other, the first recesses of the substrates jointly form a first channel, the second recesses of the substrates jointly form at least one second channel connected to the outside of the sample collection device, and the first channel and the at least one second channel are interconnected,
• wherein the surfaces of the first recess and the at least one second recess of each of the substrates are fully covered by an insulation layer;
• and
• a spacer, disposed between the two first surfaces to bond and fix the two substrates, wherein a sample containing space is formed between the two substrates and the spacer, and the sample containing space comprises the first channel and the at least one second channel.