Flow cell
AssigneeCytonome/St, LLC
InventorsJohnathan Charles Sharpe, Rudolf Hulspas, Kristopher Scott Buchanan
Life of this patent
Estimating the term…
Abstract
A flow cell for particle processing such as particle sorting, may be operatively engaged to a particle processing apparatus. The fluid contact surfaces of the flow cell may be fully enclosed. Further, the flow cell may encapsulate all fluid contact surfaces in the particle processing apparatus. The enclosing or encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The flow cell may employ any suitable technique for processing particles. The flow cell may be disposable and suitable for use in droplet sorting. The flow cell may include an operatively sealed sort chamber having a particle stream focusing region, an orifice, an interrogation zone and a sorting region.
Claims15
A flow cell for processing particles in a stream, comprising,
- an enclosed sort chamber including a first plurality of fluid contact surfaces and a second plurality of fluid contact surfaces, the first plurality of fluid contact surfaces forming a plurality of regions in fluid communication with one another and defining:
- a stream focusing region;
- an orifice located downstream of the focusing region;
- a particle interrogation region located downstream of the orifice;
- and
- a droplet deflection region;
- the second plurality of fluid contact surfaces defining a sample vessel provided upstream of the stream focusing region and in fluid communication with the stream focusing region,
- wherein the fluid contact surfaces of the sort chamber are integrally constructed,
- wherein the sort chamber includes at least one surface transmissible to electromagnetic radiation from an excitation source,
- wherein the flow cell is operatively sealed, and
- wherein the sample vessel is integrally constructed with the sort chamber.
Dependencies are inferred from the claim text.