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US 10,379,026 B2Granted
Cell processing using magnetic particles
AssigneeInguran, LLC
InventorKristie Krug
Life of this patent
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Abstract
The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.
Claims25
A method of sex-sorting sperm cells comprising the steps of:
- forming a composition comprising the sperm cells and magnetic particles, the sperm cells comprising viable sperm cells and dead or damaged sperm cells and each magnetic particle being no greater than 1000 nm in size and coated with a chargeable silicon-containing compound and comprising a negative zeta potential charge;
- binding a portion of the dead or damaged sperm cells to the magnetic particles through an electrical charge interaction;
- magnetically separating the bound dead or damaged sperm cells from the unbound viable sperm cells;
- and
- sex-sorting the separated unbound viable sperm cells, by isolating X chromosome-bearing sperm cells or Y chromosome-bearing sperm cells into a separate subpopulation of viable sex-sorted sperm cells, or by photo-damaging sperm cells bearing an undesired sex chromosome.
2 more elements
Dependencies are inferred from the claim text.