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US 10,378,055 B2Granted
Methods and compositions for measuring beta cell death
AssigneeCity of Hope
InventorsKevin Ferreri, Mohamed I. Husseiny Elsayed
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Abstract
Provided herein are, inter alia, nucleic acids, methods, and kits for detecting unmethylated DNA in body fluid sample of a subject. The disclosure includes compositions, methods, and kits for detecting unmethylation at a CpG site in an insulin gene promoter of a subject.
Claims32
A method of detecting unmethylated deoxyribonucleic acid (DNA) in a subject, the method comprising isolating DNA from a blood sample of said subject;
- contacting said isolated DNA with bisulfite for modifying unmethylated cytosine at a CpG site in said DNA to uridine;
- and detecting unmethylation of said CpG site in an insulin gene promoter located at −19 bp, −69 bp, −135 bp, −206 bp, or −357 bp relative to the transcription start site.
A system, comprising:
- at least one processor;
- and
- at least one memory including program code which when executed by the one memory provides operations comprising:
- collecting deoxyribonucleic acid (DNA) data associated with a subject;
- contacting isolated DNA from a blood sample of a subject with bisulfite for modifying unmethylated cyctosine at a CpG site in said DNA to uridine
- detecting unmethylation of DNA at said CpG site in an insulin gene promoter located at −19 bp, −69 bp, −135 bp, −206 bp, or −357 bp relative to the transcription start site;
- and
- providing, via a user interface, a prognosis and/or diagnosis for the subject based at least in part on detected unmethylated DNA.
5 more elements
Dependencies are inferred from the claim text.