Method for the regioselective deacetylation of mannosylerythritol lipids
AssigneeUniversiteit Antwerpen
InventorsEliane Yvonne Goossens, Marc Victor Henri Wijnants
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Abstract
The present invention relates to methods for the enzymatic deacetylation of mannosylerythritol lipids produced by fermentation using lipases. More in particular, the present invention relates to a method for the enzymatic deacetylation of mannosylerythritol lipids using a hydrolyzing enzyme in an organic solvent containing only low amounts of water, preferably, no water. It further provides the use of organic solvents, containing only low amounts of water, more preferably, no water, for the enzymatic deacetylation of mannosylerythritol lipids.
Claims12
A method for the enzymatic deacetylation of a mannosylerythritol lipid represented by formula (Ia), to obtain a mannosylerythritol lipid represented by formula (Id),
- the method comprising:
- incubating a mannosylerythritol lipid represented by formula (Ia), with a lipase in an organic solvent;
- wherein the organic solvent is selected from the group consisting of C2-C8 alcohols, and combinations thereof;
- and wherein the organic solvent contains less than 10% water.
A method for the enzymatic deacetylation of a mannosylerythritol lipid represented by formula (Ic), to obtain a mannosylerythritol lipid represented by formula (Id),
- the method comprising:
- incubating a mannosylerythritol lipid represented by formula (Ia), with a lipase in an organic solvent;
- wherein the organic solvent is selected from the group consisting of C2-C8 alcohols, and combinations thereof;
- and wherein the organic solvent contains less than 10% water.
Dependencies are inferred from the claim text.