Use of a N-acetylneuraminate lyase derived from the bacterium Aliivibrio salmonicida in the production of neuraminic acid and derivatives thereof
AssigneeUniversitetet I Tromsø—Norges Arktiske Universitet
InventorsInger Lin Uttakleiv Ræder, Bjørn Altermark, Man Kumari Gurung, Arne Oskar Smalås
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Abstract
The present invention provides to methods for the production of neuraminic acid or derivatives thereof under alkaline conditions and to the use of neuraminate lyases showing improved characteristics under alkaline conditions in the production of neuraminic acid or its derivatives. More particularly, the present invention employs inter alia a N-acetylneuraminate lyase which has been isolated from the psychrophilic bacterium Aliivibrio salmonicida LFI1238 and has shown to have improved characteristics under alkaline conditions.
Claims12
A method for the production of neuraminic acid or a derivative thereof comprising:
- a) transforming glucosamine or a derivative thereof into mannosamine or a derivative thereof by epimerization at an alkaline pH above 9;
- and
- b) reacting mannosamine or the derivative thereof with pyruvate at an alkaline pH of at least 9.5 in the presence of a polypeptide comprising the amino acid sequence as set forth in SEQ ID NO:
- 1 or a functional variant thereof;
- wherein the functional variant is a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 1.
A method for the production of neuraminic acid or a derivative thereof comprising:
- reacting mannosamine or a derivative thereof with pyruvate at an alkaline pH of at least 9.5 in the presence of a polypeptide comprising the amino acid sequence as set forth in SEQ ID NO:
- 1 or a functional variant thereof;
- wherein the functional variant is a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.