Tomato yellow leaf curl virus resistance
AssigneesWageningen Universiteit, University of Florida Research Foundation, Inc.
InventorsYuling Bai, Maarten Gertjan Verlaan, Samuel Hutton
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Abstract
Molecular markers are described for determining the presence or absence of a gene conferring resistance to tomato yellow leaf curl virus from S. chilense (Ty-1) in a host plant. Also described, are methods for producing a host plant comprising a gene conferring resistance to tomato yellow leaf curl virus from S. chilense (Ty-1), including the analysis of the presence or absence of the molecular markers. A plant, and parts thereof, obtained by such a method are also described.
Claims8
A method of producing a seed having resistance to tomato yellow leaf curl virus (TYLCV) in a plant of a Solanaceae family, the method comprising:
- (a) crossing genetic material comprising a region from 21.0 million base pairs (mbp) (±1 mbp) upstream the long arm of chromosome 6 to 31.8 mbp (±1 mbp) upstream the long arm of chromosome 6 from S. chilense into a host tomato plant to produce a progeny plant that comprises genetic material from S. chilense, wherein the genetic material comprises MSc05732-4 marker having SEQ ID NO:42;
- (b) backcrossing the progeny plant with the host plant to obtain a plant population;
- (c) screening for a presence of at least one Ty-1 gene in the plant population with a cleaved amplified polymorphic sequence marker (CAPS marker) that is a MSc05732-4 marker;
- (d) selecting a TYLCV-resistant plant from the plant population, wherein the TYLCV-resistant plant has expression of the at least one Ty-1 gene located between markers M067G18-1 and MSc05732-18 on chromosome 6 of the TYLCV-resistant plant;
- and
- (e) collecting a seed from the TYLCV-resistant plant, wherein the seed comprises the at least one Ty-1 gene and a phenotypic trait of TYLCV resistance.
A method of producing a tomato yellow leaf curl virus (TYLCV) resistant plant of the Solanaceae family, the method comprising:
- (a) crossing genetic material comprising a region from 21.0 million base pairs (mbp) (±1 mbp) upstream the long arm of chromosome 6 to 31.8 mbp (±1 mbp) upstream the long arm of chromosome 6 from S. chilense into a host plant, wherein the host plant of the Solanaceae family is a tomato plant, wherein the host plant is a tomato yellow leaf curl virus-susceptible plant, to produce a progeny plant that comprises genetic material from S. chilense;
- (b) backcrossing the progeny plant with the host plant to obtain a plant population;
- (c) screening for a presence of at least one Ty-1 resistant allele in the plant population with a cleaved amplified polymorphic sequence marker (CAPS marker) that is a MSc05732-4 marker;
- (d) selecting a TYLCV-resistant plant from the plant population, wherein the TYLCV-resistant plant has expression of at least one Ty-1 gene located between markers M067G18-1 and MSc05732-18 on chromosome 6 of the TYLCV-resistant plant;
- (e) collecting a seed from the TYLCV-resistant plant;
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- (f) regenerating the seed to obtain a tomato yellow leaf curl virus (TYLCV) resistant plant comprising the at least one Ty-1 gene and a phenotypic trait of TYLCV resistance.
Dependencies are inferred from the claim text.