Compositions and methods for controlling insect pests
AssigneeMonsanto Technology LLC
InventorsMichael John Crawford, Brian Donovan Eads
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Abstract
Disclosed herein are polynucleotides, compositions, and methods for controlling insect pests, especially flea beetles, such as Phyllotreta spp. and Psylliodes spp., particularly in plants. More specifically, polynucleotides such as double-stranded RNA triggers and methods of use thereof for modifying the expression of genes in flea beetles.
Claims16
A method of causing mortality or stunting in an insect, comprising contacting an insect with at least one recombinant double-stranded RNA (dsRNA) comprising at least one silencing element comprising an RNA strand identical or complementary to 21 or more contiguous nucleotides of an insect target gene sequence that has a DNA sequence of SEQ ID NO:
- 327, and the complement thereof, wherein said insect is Phylotreta spp., and wherein ingestion of said recombinant dsRNA by said insect results in mortality or stunting in said insect.
An insecticidal composition comprising an insecticidally effective amount of a recombinant dsRNA molecule, wherein said recombinant dsRNA molecule comprises an RNA strand complementary to 21 or more contiguous nucleotides of a target gene of an insect that infests a plant, and the complement thereof, wherein said insect is Phylotreta spp., and wherein said target gene has a DNA sequence of SEQ ID NO:
- 327.
A recombinant DNA construct comprising a heterologous promoter operably linked to DNA encoding a dsRNA comprising a sequence identical or complementary to 21 or more contiguous nucleotides of SEQ ID NO:
- 1186, and the complement thereof.
Dependencies are inferred from the claim text.