Near-infrared ray exposure system for biological studies

An apparatus comprising:

• a light source;
• a cell culture plate configured to fix thereon a biological sample;
• a sample stage having fixed thereon the cell culture plate and configured to provide heat to adjust a temperature of the sample;
• a microscope apparatus capable of capturing and processing real-time images of the sample as it is subjected to the light emitted from the light source, the microscope apparatus comprising a microscope selected from an electron microscope and a scanning probe microscope;
• a temperature-sensing device connected to the sample stage and measuring a temperature of the sample stage;
• a magnetic coil configured to provide the sample stage with a magnetic force;
• and
• an optical fiber connecting the light source to the microscope apparatus,
• the light source and the microscope being disposed above the same top surface of the sample stage, and the light source being configured such that it serves as an incident light of the microscope,
• the magnetic coil being disposed below the sample stage,
• the temperature-sensing device being configured to monitor the real-time temperature of the sample as it is fixed on or with the sample stage,
• the temperature-sensing device being selected from a thermometer, a thermistor, and a thermocouple,
• the light source being positioned to directly face an upper surface of the cell culture plate,
• the microscope apparatus further comprising computer hardware and software capable of capturing and processing real-time images of the biological sample, and
• the light source being a near-infrared (NIR) light source, and the NIR light of the NIR light source having a wavelength in a range of from 700 nm to 1000 nm, and the NIR light having a power density in a range of from 1 W/cm2 to 10 W/cm2.