Ionic liquid-tolerant cellulase enzymes
AssigneesNational Technology & Engineering Solutions of Sandia, LLC, The Regents of the University of California
InventorsJohn Gladden, Joshua Park, Steven Singer, Blake Simmons, Ken Sale
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Abstract
The present invention provides ionic liquid-tolerant cellulases and method of producing and using such cellulases. The cellulases of the invention are useful in saccharification reactions using ionic liquid treated biomass.
Claims10
A composition comprising (i) an isolated ionic liquid-tolerant cellulase having β-glucosidase activity and comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:4;
- and (ii) an ionic liquid, wherein the ionic liquid has an imidazolium cation.
A method of increasing the yield of soluble sugar from a lignocellulosic biomass, the method comprising incubating lignocellulosic biomass with a composition comprising an ionic liquid-tolerant cellulase and an ionic liquid having an imidazolium cation in an enzymatic hydrolysis reaction, wherein the ionic liquid-tolerant cellulase has β-glucosidase activity and comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:4.
Dependencies are inferred from the claim text.