Genetically engineered Vibrio sp. and uses thereof
AssigneeSynthetic Genomics, Inc.
InventorsMatthew T Weinstock, Christopher M. Wilson, Eric D. Hesek
Life of this patent
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Abstract
The present disclosure relates to the seminal discovery of a generation and use of genetically engineered Vibrio sp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and/or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery of Vibrio sp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.
Claims19
A vector operable in a Vibrio sp., comprising:
- a Vibrio chromosome II sequence comprising at least 90% sequence identity to SEQ ID NO:
- 1;
- at least one selectable marker;
- and
- a heterologous DNA sequence of greater than 10 kb.
A method of producing a biomolecule comprising:
- a) contacting Vibrio sp. bacteria with a vector comprising a heterologous nucleic acid encoding the biomolecule and further comprising SEQ ID NO:
- 1 or a variant thereof, such that the heterologous nucleic acid is introduced into the bacteria;
- b) growing the bacteria in a growth-conducive medium wherein the heterologous nucleic acid is expressed, thereby producing the biomolecule;
- and
- c) isolating the biomolecule.
Dependencies are inferred from the claim text.