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US 10,377,995 B2Granted
Mutated genes for the catalytic protein of Oplophorus luciferase and use thereof
AssigneeJnc Corporation
InventorsSatoshi Inouye, Yuiko Miura, Junichi Sato
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Abstract
A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.
Claims7
A polynucleotide comprising a nucleotide sequence encoding a recombinant luciferase mutant of (a) or (b) below:
- (a) a recombinant luciferase mutant comprising the amino acid sequence of SEQ ID NO:
- 2 substituted at the amino acid positions consisting of:
- glutamic acid at position 4;
- arginine at position 11;
- leucine at position 18;
- and
- valine at position 27;
- or
- (b) a recombinant luciferase mutant comprising the amino acid sequence of SEQ ID NO:
- 2 substituted at the positions consisting of:
- glutamic acid at position 4;
- arginine at position 11;
- leucine at position 18;
- valine at position 27;
- and
- one or more amino acid(s) at position(s) other than positions 4, 11, 18, 27, 33, 43, 44, 54, 68, 72, 75, 90, 115, 124, 138, and 166 of the amino acid sequence of SEQ ID NO:
- 2, wherein the recombinant luciferase mutant has luciferase activity.
14 more elements
Dependencies are inferred from the claim text.