Use of the reductive glycine pathway for generating formatotrophic and autotrophic microorganisms
AssigneeYeda Research and Developmetn Co. Ltd.
InventorsArren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
Life of this patent
Estimating the term…
Abstract
An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.
Claims19
An isolated microoranism that is genetically modified to overexpress at least one enzyme of the glycine cleavage system, said enzyme being selected from the group consisting of EC 2.1.2.10, EC1.8.1.4 and EC 1.4.4.2, wherein the microorganism is able to utilize formate as a carbon source for the generation of pyruvate or glycerate via the formation of glycine and serine.
An isolated microorganism that is genetically modified to express enzymes of the reductive glycine pathway, wherein the microorganism is capable of converting formate to a metabolite of central metabolism via the formation of glycine and without the formation of serine, said metabolite being selected from the group consisting of acetyl CoA, oxaloacetate, glycerate 2-phosphate and glycerate 3-phosphate, wherein said microorganism uses formate as its carbon source.
A method of generating a microorganism comprising overexpressing in the microorganism at least one enzyme of the glycine cleavage system, said enzyme being selected from the group consisting of EC 2.1.2.10, EC1.8.1.4 and EC 1.4.4.2, wherein the microorganism is capable of utilizing formate as a carbon source for the generation of pyruvate or glycerate via the formation of glycine and serine.
Dependencies are inferred from the claim text.