Recombinant microorganism and method for producing a substance using the same
AssigneesToyota Jidosha Kabushiki Kaisha, Kyoto University
InventorsChikara Ohto, Masayoshi Muramatsu, Masakazu Ito, Jun Ogawa, Shigenobu Kishino
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Abstract
This invention provides a recombinant microorganism into which an acyl-CoA reductase exhibiting excellent activity in a reduction reaction using acyl-CoA as a substrate has been introduced. Such recombinant microorganism comprises a nucleic acid encoding an acyl-CoA reductase comprising any of characteristic Common sequences 1 to 3 introduced into a host microorganism.
Claims11
A recombinant microorganism comprising a nucleic acid encoding an acyl-CoA reductase, wherein the acyl-CoA reductase is selected from (a) or (b) below:
- (a) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34;
- or
- (b) an amino acid sequence having 80% or higher identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34, and having acyl-CoA reductase activity,
- wherein said recombinant microorganism further comprises aldehyde decarbonylase activity for synthesizing a hydrocarbon using an aldehyde as a substrate.
A recombinant microorganism comprising a nucleic acid encoding an acyl-CoA reductase, wherein the acyl-CoA reductase is selected from (a) or (b) below:
- (a) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34;
- or
- (b) an amino acid sequence having 80% or higher identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34, and having acyl-CoA reductase activity,
- wherein said recombinant microorganism further comprises a nucleic acid encoding an aldehyde decarbonylase that synthesizes a hydrocarbon using an aldehyde as a substrate.
A recombinant microorganism comprising a nucleic acid encoding the protein (a) or (b) below introduced into a host microorganism:
- (a) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34;
- or
- (b) a protein comprising an amino acid sequence having 80% or higher identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34, and having acyl-CoA reductase activity,
- wherein said recombinant microorganism further comprises aldehyde decarbonylase activity for synthesizing a hydrocarbon using an aldehyde as a substrate.
A recombinant microorganism comprising a nucleic acid encoding the protein (a) or (b) below introduced into a host microorganism:
- (a) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34;
- or
- (b) a protein comprising an amino acid sequence having 80% or higher identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 and 34, and having acyl-CoA reductase activity,
- wherein said recombinant microorganism further comprises a nucleic acid encoding an aldehyde decarbonylase that synthesizes a hydrocarbon using an aldehyde as a substrate.
Dependencies are inferred from the claim text.