Method for producing a leukocyte preparation
AssigneesArtcline GmbH, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
InventorsJens Altrichter, Steffen Mitzner, Antje Lübcke, Kathleen Selleng, Fanny Doss, Stephanie Koch
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Abstract
A method for preparing a leukocyte preparation from a leukocyte fraction and a correspondingly prepared or obtainable leukocyte preparation and its use. The method includes: a) sedimenting the leukocyte fraction removing leukocyte supernatant from the sediment, wherein the leukocyte supernatant is collected or remains in a leukocyte container, b) sedimenting the leukocytes in the leukocyte container, c) washing the sediment in the leukocyte container with a saline solution, and d) resuspending the sediment in the leukocyte container in a storage solution.
Claims17
A method for preparing a leukocyte preparation from a leukocyte fraction, the method comprising:
- a) sedimenting the leukocyte fraction until a dividing line has formed between the sedimented erythrocytes and a leukocyte supernatant, removing the leukocyte supernatant from the sediment, wherein the leukocyte supernatant is collected or remains in a leukocyte container;
- b) sedimenting the leukocytes in the leukocyte container, wherein a low-leukocyte supernatant is formed, and removing the low-leukocyte supernatant from the leukocyte container;
- c) washing the sediment in the leukocyte container with a saline solution, wherein the sedimented cells are resuspended in the saline solution, the solution is sedimented and a resulting supernatant is removed;
- d) resuspending the sediment in the leukocyte container in a storage solution;
- and
- e) storing the solution from step d) in a gas-permeable bag at a temperature between 20° C. and 25° C. for at least 48 hours.
Dependencies are inferred from the claim text.