Three-dimensional silk fibroin scaffold culture retaining functional salivary cells and promoting salivary tissue-specific ECM synthesis
AssigneeBoard of Regents, The University of Texas System
InventorsXiao-Dong Chen, Chih-ko Yeh, Joo L. Ong
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Abstract
A cell culture system including a silk fibroid scaffold, culture media, and salivary gland cells. The salivary gland cells grown in the tissue culture system have physiological and morphological features like those of in vivo salivary gland cells. The cell culture system can be used to produce a salivary tissue-specific extracellular matrix capable of inducing differentiation of salivary gland cell precursors into salivary gland cells.
Claims11
A method of forming a salivary tissue-specific extracellular matrix comprising:
- culturing salivary gland cells on a silk fibroin scaffold;
- and
- exposing the salivary gland cells to ascorbic acid.
A method of producing differentiated salivary gland cells, the method comprising:
- (a) generating a three-dimensional extracellular matrix by culturing salivary gland cells on a silk fibroin scaffold;
- (b) decellularizing the extracellular matrix;
- (c) incubating precursors of salivary gland cells with the decellularized three-dimensional extracellular matrix to produce differentiated salivary gland cells.
Dependencies are inferred from the claim text.