Cell culture medium and culture method using the same
AssigneesKaneka Corporation, National Hospital Organization
InventorsTomohisa Kato, Yonehiro Kanemura, Tomoko Shofuda, Hayato Fukusumi
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Abstract
It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular which does not comprise serum. The present invention provides a cell culture medium which comprises growth arrest-specific 6 (GAS6) and does not comprise serum.
Claims7
A method for culturing cells, said method comprising:
- culturing cells using a cell culture medium, wherein the cell culture medium comprises growth arrest-specific 6 (GAS6), a basal medium, and at least one selected from the group consisting of decorin, matrix metalloproteinase-3 (MMP3), osteopontin (OPN), TNF-related weak inducer of apoptosis receptor (TWEAK R), insulin-like growth factor-binding protein 2 (IGFBP2), galectin 1 (LGALS1), and insulin-like growth factor-1 (IGF-1),
- wherein the cell culture medium does not comprise serum, and the concentration of the GAS6 contained in the cell culture medium is between 2 ng/ml and 100 ng/ml, and
- wherein the cells are cultured on a culture substrate having a surface that has not been coated with an extracellular matrix.
A method for culturing cells, said method comprising:
- culturing cells using a cell culture medium, wherein the cell culture medium comprises growth arrest-specific 6 (GAS6), a basal medium, and at least one selected from the group consisting of decorin, matrix metalloproteinase-3 (MMP3), osteopontin (OPN), TNF-related weak inducer of apoptosis receptor (TWEAK R), insulin-like growth factor-binding protein 2 (IGFBP2), galectin 1 (LGALS1), and insulin-like growth factor-1 (IGF-1),
- wherein the cell culture medium does not comprise serum, and the concentration of the GAS6 contained in the cell culture medium is between 2 ng/ml and 100 ng/ml, and
- wherein the cells that are cultured are iPS cells (induced pluripotent stem cells).
A method for culturing cells, which comprises culturing cells using a cell culture medium which comprises growth arrest-specific6 (GAS6), decorin, matrix metalloproteinase-3 (MMP3), osteopontin (OPN), TNF-related weak inducer of apoptosis receptor (TWEAK R), insulin-like growth factor-binding protein 2 (IGFBP2), galectin 1 (LGALS1), and insulin-like growth factor-1 (GF-1), does not comprise serum, and is not conditioned with MEF.
Dependencies are inferred from the claim text.