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US 10,377,983 B2Granted
Methods for the production of diatom biomass
AssigneeFermentalg
InventorsMark Humphrey Dines, Hywel David Griffiths, Moreland David Gibbs
Life of this patent
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Abstract
The invention relates to methods of producing a diatom biomass. More particularly, the invention relates to methods to produce a diatom biomass using a continuous culture to produce a volumetric production rate of biomass of at least 20 g dry weight/L/day, wherein the culture medium is designed to provide the essential nutrients to maintain the diatom in log phase growth. In a preferred cmbodimcnt the biomass comprises highly unsaturated fatty acid.
Claims9
A method of producing at least one highly unsaturated fatty (HUFA) or esters thereof, the method comprising the steps of:
- i) producing a diatom biomass by continuously culturing a diatom in a culture medium, wherein the volumetric production rate of biomass is at least 20 g dry weight/L/day, and wherein the dilution rate of the culture medium maintains the diatom in log phase growth,
- ii) subjecting the diatom biomass to one or more extraction steps to extract the at least one HUFA or esters thereof from the biomass, thereby yielding an extract and
- iii) purifying the at least one HUFA or esters thereof from the extract.
Dependencies are inferred from the claim text.