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US 10,377,794 B2Granted
Optimized method for antibody capturing by mixed mode chromatography
AssigneeHoffmann-La Roche Inc.
InventorsRoberto Falkenstein, Thorsten Lemm, Markus Strasser, Hidenari Yamada
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Abstract
Herein is reported a method for the purification of an antibody directly captured from clarified cell culture supernatants using Streamline CST and/or Capto MMC, wherein especially product related (aggregates and fragments) and process related impurities (host cell protein, media components) could efficiently be removed, resulting in a preparation with a purity comparable to classical protein A affinity chromatography.
Claims11
A method for producing an antibody comprising the following steps:
- a) applying a crude mammalian cell culture cultivation supernatant to a multimodal weak cation exchange chromatography material;
- and
- b) recovering the antibody by applying a buffered solution comprising ethylene glycol and an inorganic salt to the multimodal weak cation exchange chromatography material, thereby producing the antibody.
Dependencies are inferred from the claim text.