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US 10,377,792 B2Granted
Moisture displacement and simultaneous migration of surface-functionalized algae from water to an extraction solvent using ionic polyelectrolytes
AssigneeThe Texas A&M University System
InventorsSandun D. Fernando, Varun M. Gejji
Life of this patent
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Abstract
This invention is in the field of micro-organism and algal cell processing. The invention relates to a method of maximizing migration of micro-organism and/or algal cells to a solvent fraction while simultaneously displacing water in a separate fraction and subsequent extraction of hydrophobic products from the organisms. The invention further relates to a method of sequestration of protein from an aqueous phase to an organic solvent.
Claims11
A method for the extraction of algal oil comprising:
- a) providing:
- i) algae cells comprising algal oil and a hydrophilic cell surface;
- ii) an amphiphilic agent capable of converting said hydrophilic cell surface to a hydrophobic cell surface, wherein said amphiphilic agent is selected from the group consisting of:
- Poly(diallyldimethylammonium chloride), Poly(sodium 4-styrenesulfonate), Poly(3-(1-Pyridinio)-1-propanesulfonate), a monomeric form of at least one of the group consisting of diallyldimethylammonium chloride, sodium 4-styrenesulfonate, and 3-(1-Pyridinio)-1-propanesulfonate, and an oligomeric form of at least one of the group consisting of diallyldimethylammonium chloride, sodium 4-styrenesulfonate, and 3-(1-Pyridinio)-1-propanesulfonate;
- and
- iii) an extractant;
- b) adding said amphiphilic agent to said algae cells under conditions such that said hydrophobic cell surface is created, and
- c) purifying said algal oil with said extractant.
5 more elements
A method for the extraction of protein comprising:
- a) providing:
- i) an aqueous solution comprising multi-component suspension, said multi-component suspension comprising hydrophilic protein;
- ii) an amphiphilic agent capable of converting said hydrophilic protein to a hydrophobic protein, wherein said amphiphilic agent is selected from the group consisting of:
- Poly(diallyldimethylammonium chloride), Poly(sodium 4-styrenesulfonate), Poly(3-(1-Pyridinio)-1-propanesulfonate), a monomeric form of at least one of the group consisting of diallyldimethylammonium chloride, sodium 4-styrenesulfonate, and 3-(1-Pyridinio)-1-propanesulfonate, and an oligomeric form of at least one of the group consisting of diallyldimethylammonium chloride, sodium 4-styrenesulfonate, and 3-(1-Pyridinio)-1-propanesulfonate;
- and
- iii) an extractant;
- b) adding said amphiphilic agent to said multi-component suspension, and
- c) purifying said protein with said extractant.
5 more elements
Dependencies are inferred from the claim text.