US 10,377,787 B2 · Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation

Abstract

The present application discloses a simple process for the purification of neutral human milk oligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of a cationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and/or electrodialysis step, which allows efficient purification of large quantities of neutral HMOs at high purity. Contrary to the purification currently used in fermentative production of neutral HMOs, the presented process allows the provision of HMOs without the need of a chromatographic separation. The so purified HMOs may be obtained in solid form by spray drying, as crystalline material or as sterile filtered concentrate. The provided HMOs are free of proteins and recombinant material originating from the used recombinant microbial strains and thus very well-suited for use in food, medical food and feed (e.g. pet food) applications.

Claims26

Dependent claims

A process for the purification of 2′-fucosyllactose in a batch manner or in a continuous manner from a fermentation broth obtained by microbial fermentation, wherein the fermentation broth comprises kilogram amounts of 2′-fucosyllactose, which process comprises

i) separating the microbial biomass from the fermentation broth;
ii) subjecting the separated fermentation broth obtained in step i) to a cation exchanger or to an anion exchanger to obtain a solution;
iii) subjecting the solution obtained in step ii) to the cation or anion exchanger not used in step ii);
iv) subjecting the solution obtained from step iii) to nanofiltration or reverse osmosis or vacuum evaporation to obtain a purified solution of 2′-fucosyllactose;
v) optionally treating the purified solution obtained from step iv) with activated carbon;
wherein the 2′-fucosyllactose in the purified solution obtained after step iv) or step v) has a purity of ≥80% as determined by HPLC.

3 more elements

Dependencies are inferred from the claim text.

Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation

Life of this patent

Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation

Life of this patent

Abstract

Claims26