Devices, apparatus, kit and method for treating a biological sample
AssigneeMenarini Silicon Biosystems S.p.A.
InventorsAlex Calanca, Gianni Medoro, Nicolò Manaresi, Giuseppe Giorgini
Life of this patent
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Abstract
Method for the treatment of a biological sample comprising at least one cell and a liquid component; according to the method, a force is applied to the sample inserted in an inner chamber of a hollow device towards a filter which has pores with diameters from 2 nm to 1 μm, so that at least part of the liquid component passes through the filter and the cell remains in the inner chamber, thus obtaining a concentrated sample; the filter has a surface facing the inner chamber of less than 12.6 mm2.
Claims23
A hollow device for treating a biological sample by separating at least a particle and at least part of a liquid from each other, comprising:
- an inner chamber, which has a volume up to 2 mL;
- a first end;
- a first opening, which is arranged in the area of the first end, and establishes access to the inner chamber from outside the inner chamber, and the first opening has an area of at least 9 mm2;
- a second end defining a second opening, wherein the liquid flows out from the inner chamber through the second opening;
- and
- a filter, which is arranged in the area of the second end and extends across a surface of the second opening exterior to the inner chamber, separates the inner chamber from the outside, has pores with diameters ranging from 2 nm to 1 μm, an area facing the inner chamber of up to 12.6 mm2, and a thickness up to 500 μm;
- and
- at least one wall, which delimits the inner chamber and has a thermal conductivity from 0.08 W/mK to 0.7 W/mK, wherein the at least one wall has a thickness up to 700 μm in at least a region of the at least one wall upstream of the filter and the second end.
An apparatus for treating a sample, comprising:
- a hollow device, comprising:
- an inner chamber having a volume of up to 2 mL,
- a first end,
- a first opening arranged in the area of the first end and providing communication between the inner change and the outside, wherein the opening has an area of at least 9 mm2,
- a second end
- at least one wall, which delimits the inner chamber and has a thermal conductivity from 0.08 W/mK to 0.7 W/mK and a thickness up to 700 μm, and
- a filter arranged in the area of the second end, the filter separating the inner chamber from the outside, the filter comprising pores having diameters ranging from 2 nm to 1 μm, an area facing the inner chamber up to 12.6 mm2, and a thickness of up to 500 μm;
- and
- a covering device configured to externally couple to the hollow device and substantially obstruct the filter and prevent material from passing from the inner chamber to the outside through the filter when coupled to the hollow device, the covering comprising:
- a cavity comprising an open end sized to allow the hollow device to be inserted into the cavity, and a closed end′ and
- an adjustment element that adjusts to the shape of the hollow device when the covering device is coupled to the hollow device.
A hollow device for treating a biological sample by separating at least a particle and at least part of a liquid from each other, comprising:
- one or more walls that cooperate to define an inner chamber extending between oppositely disposed first and second ends;
- a first opening defined in the first end, the first opening establishing access to the interior chamber from outside the chamber, wherein the first opening has an area of at least 9 mm2;
- a second opening defined in the second end;
- and
- a filter extending across the second opening on a surface exterior to the inner chamber, thereby separating the inner chamber from the outside, wherein:
- the inner chamber has a volume up to 2 mL,
- the one or more walls have a uniform thickness of up to 700 μm and a thermal conductivity of 0.08 W/mK to 0.7 W/mK, and
- the filter has pores with diameters ranging from 2 nm to 1 μm, an area facing the inner chamber of up to 12.6 mm2, and a thickness up to 500 μm.
Dependencies are inferred from the claim text.