Nanoplatforms for arginase, indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase detection by posttranslational modification

A nanoplatform assembly for detecting the presence of an active enzyme in a sample, wherein said enzyme is indoleamine 2,3-dioxygenase 1, said nanoplatform comprising:

• a central carrier particle;
• a first particle;
• a second particle;
• and
• an oligopeptide linkage connecting said central carrier particle and said second particle,
• wherein said first particle is directly attached to said central carrier particle, and wherein said oligopeptide linkage comprises a substrate sequence for said enzyme, said substrate sequence comprising a sequence according to (III) or (IV):
• (X)n(W)m(X)l (SEQ ID NO:3), (III)
• where:
• each W:
• L- or D-tryptophan;
• each X:
• any amino acid;
• m:
• 3-10
• n:
• 1-6;
• and
• l:
• 1-6;
• or
• (X)n(WU)m(X)l (SEQ ID NO:4), (IV)
• where:
• each W:
• L- or D-tryptophan;
• each X:
• any amino acid;
• each U:
• G, N, D, P, or S;
• m:
• 2-20;
• n:
• 1-6;
• and
• l:
• 1-6.

A nanoplatform assembly for detecting the presence of an active enzyme in a sample, wherein said enzyme is tryptophan 2,3-dioxygenase, said nanoplatform comprising:

• a central carrier particle;
• a first particle;
• a second particle;
• and
• an oligopeptide linkage connecting said central carrier particle and said second particle,
• wherein said first particle is directly attached to said central carrier particle, and wherein said oligopeptide linkage comprises a substrate sequence for said enzyme, said substrate sequence comprises a sequence according to (III) and/or (IV):
• (X)n(W)m(X)l (SEQ ID NO:3), (III)
• where:
• each W:
• L-tryptophan;
• each X:
• any amino acid;
• m:
• 3-10
• n:
• 1-6;
• and
• l:
• 1-6;
• or
• (X)n(WU)m(X)l (SEQ ID NO:4), (IV)
• where:
• each W:
• L-tryptophan;
• each X:
• any amino acid;
• each U:
• G, N, D, P, or S;
• m:
• 2-20;
• n:
• 1-6;
• and
• l:
• 1-6.