Multiplexed genome editing
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Luhan Yang, Marc Guell
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Abstract
A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.
Claims19
A method of altering porcine endogenous retrovirus (PERV) pol genes in a cell comprising:
- introducing into the cell a Cas protein and guide RNAs, wherein each guide RNA comprises a sequence that is complementary to a portion of a PERV pol gene;
- and
- maintaining the cell under conditions in which the guide RNAs and the Cas protein form co-localization complexes with a plurality of PERV pol genes and the Cas protein cuts at least 20% of the PERV pol genes.
Dependencies are inferred from the claim text.