# US 10,371,610 B2 · Contact-type patch, staining method using the same, and manufacturing method thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,371,610 B2 - Document ID: US10371610B2 - Status: Granted - Kind: B2 - Application number: 15/206,247 - Filed: 2016-07-09 - Published: 2017-08-24 - Granted: 2019-08-06 - Earliest claimed: 2016-02-23 - Assignees: Noul Co., Ltd. - Applicants: Noul Co., Ltd. - Inventors: Dongyoung Lee; Kyunghwan Kim; Youngmin Shin; Hyunjeong Yang; Chanyang Lim - Web page: https://patentgrep.com/patent/US10371610B2 ## CPC - G01N 1/31: Apparatus therefor - G01N 1/30: Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} - G01N 1/312: {for samples mounted on planar substrates} - G01N 2001/302: {Stain compositions} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present disclosure relates to a gel-phase patch that performs a function of assisting in staining during a staining process such as a process of coming into contact with a specimen such as blood to perform a staining function of staining the specimen, a process of fixing the specimen, or a process of forming an optimal pH at a specimen stained by a staining sample. According to an aspect of the present disclosure, a contact-type staining patch includes a staining solution that reacts with a specimen and a gel receptor provided as a gel matrix of a mesh structure in which a pore that accommodates the staining solution is formed and the mesh structure prevents the staining solution in the pore from leaking or degenerating, and having a contact surface that comes into contact with the specimen to transfer some of the staining solution to the specimen. ## Claims ### Claim 1 A specimen preparation kit for a disease diagnosis comprising: a contact-type staining patch including a staining solution and a gel receptor, wherein the staining solution has a detection inducing substance configured to react with a specimen to be inspected, and the contact-type staining patch has a contact surface that comes into contact with the specimen to transfer the detection inducing substance; a patch storing member that supports and moves with the contact-type staining patch; and a guide wall that guides the patch storing member to move at least in a vertical direction, which is vertical to a plane on which the specimen is placed, wherein the plane is provided by a specimen receiving member on which the specimen is placed; wherein the patch storing member is configured to be movably connected to the specimen receiving member, wherein the patch storing member moves along the guide wall relative to the specimen receiving member in the vertical direction, and wherein the gel receptor is provided as a gel matrix of a mesh structure in which a plurality of pores that contain the staining solution is formed and the mesh structure is configured to prevent the staining solution in the plurality of pores from leaking or degenerating. ### Claim 2 (depends on 1) The specimen preparation kit of claim 1, wherein: when the patch storing member is at a first position relative to the specimen receiving member, the contact-type staining patch is in contact with the specimen so that the detection staining solution is transferred to the specimen and when the patch storing member is at a second position relative to the specimen receiving member, the contact-type staining patch is separated from the specimen. ### Claim 3 (depends on 1) The specimen preparation kit of claim 1, wherein: when the guide wall is connected with the specimen receiving member, the guide wall is configured to not move in the vertical direction relative to the specimen. ### Claim 4 (depends on 1) The specimen preparation kit of claim 1, wherein: the patch storing member is configured to be directly connected to the specimen receiving member. ### Claim 5 (depends on 1) The specimen preparation kit of claim 1, wherein: the guide wall is extended from the specimen receiving member in a direction that is perpendicular to the plane. ### Claim 6 (depends on 1) The specimen preparation kit of claim 1, wherein: the guide wall is fixed to the specimen receiving member. ### Claim 7 (depends on 1) The specimen preparation kit of claim 1, wherein: the detection inducing substance is configured to visually label a target component in the specimen. ### Claim 8 (depends on 1) The specimen preparation kit of claim 1, wherein: the patch storing member contacts with an upper surface that is provided on the opposite side of the contact surface to support the contact-type staining patch. ### Claim 9 (depends on 1) The specimen preparation kit of claim 1, wherein: the contact-type staining patch is interposed between the plane and the patch storing member. ### Claim 10 (depends on 1) The specimen preparation kit of claim 1, wherein: the guide wall is spaced from the contact-type staining patch. ### Claim 11 (depends on 1) The specimen preparation kit of claim 1, wherein: the guide wall includes a guide rail that is formed on the guide wall and provides a path for the patch storing member to move in the vertical direction relative to the plane. ### Claim 12 (depends on 1) The specimen preparation kit of claim 1, wherein: the patch storing member contacts with a side surface of the contact-type staining patch, wherein the side surface is adjacent to the contact surface of the contact-type staining patch. ### Claim 13 (depends on 1) The specimen preparation kit of claim 1, wherein: the patch storing member covers an upper surface of the contact-type staining patch on an opposite side of the contact surface. ### Claim 14 A specimen preparation kit for a disease diagnosis comprising: a contact-type staining patch including a staining solution and a gel receptor, wherein the staining solution is configured to stain a specimen to be inspected placed on a specimen receiving member, and wherein the contact-type staining patch has a contact surface that comes into contact with the specimen to transfer a part of the staining solution to the specimen and an upper surface provided on an opposite side of the contact surface; and a patch storing member which supports and moves with the contact-type staining patch, wherein the patch storing member contacts with the upper surface of the contact-type staining patch to support the contact-type staining patch; wherein the gel receptor is provided as a gel matrix of a mesh structure in which a plurality of pores that contains the staining solution is formed, wherein the patch storing member is configured to be movably connected to the specimen receiving member. ### Claim 15 (depends on 14) The specimen preparation kit of claim 14, wherein: the patch storing member further includes a guide formed on a side surface of the patch storing member. ### Claim 16 (depends on 15) The specimen preparation kit of claim 15, wherein: the guide is formed on the side surface that is not in contact with the contact-type staining patch. ### Claim 17 (depends on 15) The specimen preparation kit of claim 15, wherein: the guide is configured to guide the patch storing member to move relative to the specimen receiving member. ### Claim 18 (depends on 15) The specimen preparation kit of claim 15, wherein: the guide guides the patch storing member to move in vertical direction relative to the specimen receiving member. ### Claim 19 (depends on 15) The specimen preparation kit of claim 15, wherein: the guide guides the patch storing member to move between a first position and a second position relative to the specimen receiving member, when the patch storing member is at the first position, the contact-type staining patch is in contact with the specimen, and when the patch storing member is at the second position, the contact-type staining patch is separated from the specimen. ### Claim 20 (depends on 14) The specimen preparation kit of claim 14, wherein: when the patch storing member is at a first position relative to the specimen, the contact-type staining patch is in contact with the specimen so that the staining solution is provided to the specimen, and when the patch storing member is at a second position relative to the specimen, the contact-type staining patch is separated from the specimen.