# US 10,370,712 B2 · Signatures of radiation response

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- Number: US 10,370,712 B2
- Document ID: US10370712B2
- Status: Granted
- Kind: B2
- Application number: 14/430,635
- Filed: 2013-09-24
- Published: 2015-09-10
- Granted: 2019-08-06
- Earliest claimed: 2012-09-24
- Assignees: Duke University
- Applicants: Duke University
- Inventors: John P. Chute; Joseph R. Nevins; Joseph Lucas; Holly K. Dressman
- Web page: https://patentgrep.com/patent/US10370712B2

## CPC

- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q 1/6813: Hybridisation assays
- C12Q 2600/158: Expression markers
- C12Q 2600/16: Primer sets for multiplex assays
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates, in general, to gene expression profiles, and in particular, to a gene expression profile of an environmental exposure, ionizing radiation. The invention further relates to methods of screening patients for radiation exposure based on gene expression profiling and to kits suitable for use in such methods.

## Claims

### Claim 1

A method comprising:
i) obtaining a blood sample from a human,
ii) isolating mononuclear cells from said blood sample,
iii) extracting RNA from said mononuclear cells,
iv) analyzing RNA in said blood sample for expression of a gene set consisting of FDXR, ASPA, RFC4, METTL8, RASL12, ASTN2, RASA4, TRIB2, BBC3, RPA1, Gna15, H2AFV, PARP1, CEBPB, CDKN1A, PRIM1, NINJ1, BAX, HIST1H3D, HIST1H2BH, DDB2, BCL11B, FAM134C, and LAPTM5, or a subset thereof comprising at least 5 of said genes and comprising TRIB2, PARP1, and LAPTM5; and wherein said blood sample is obtained within 7 days of exposure of said individual to radiation.

### Claim 2 (depends on 1)

The method according to claim 1 wherein said blood sample is a peripheral blood sample.

### Claim 3 (depends on 1)

The method according to claim 1 wherein said blood sample is obtained at about 24-168 hours after exposure of said individual to radiation.

### Claim 4 (depends on 1)

The method according to claim 1 wherein said extracted RNA resulting from step (iii) is amplified.

### Claim 5

A method comprising:
i) obtaining a blood sample from a human, and
ii) analyzing RNA in the blood sample for expression of a gene set consisting of FDXR, ASPA, RFC4, METTL8, RASL12, ASTN2, RASA4, TRIB2, BBC3, RPA1, Gna15, H2AFV, PARP1, CEBPB, CDKN1A, PRIM1, NINJ1, BAX, HIST1H3D, HIST1H2BH, DDB2, BCL11B, FAM134C, and LAPTM5, or a subset thereof comprising at least 5 of said genes and comprising TRIB2, PARP1, and LAPTM5; and wherein said blood sample is obtained within 7 days of exposure of said individual to radiation.

### Claim 6 (depends on 5)

The method according to claim 5 wherein said blood sample is obtained at about 24-168 hours after exposure of said individual to radiation.