# US 10,370,694 B2 · Size dependence of nanoparticle-protein interaction and high sensitivity potentiometric sensor exploiting such interactions

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- Number: US 10,370,694 B2
- Document ID: US10370694B2
- Status: Granted
- Kind: B2
- Application number: 15/156,648
- Filed: 2016-05-17
- Published: 2016-11-24
- Granted: 2019-08-06
- Earliest claimed: 2015-05-19
- Assignees: New York University
- Applicants: New York University
- Inventors: Kalle Levon; Yanyan Wang
- Web page: https://patentgrep.com/patent/US10370694B2

## CPC

- C12Q 1/006: {for glucose}
- C12Q 1/003: {Functionalisation}
- C25D 3/48: of gold
- C25D 5/18: Electroplating using modulated, pulsed or reversing current
- C25D 5/54: Electroplating of non-metallic surfaces (C25D7/12 takes precedence)
- G01N 27/3278: {involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745)}
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- G01N 2333/904: acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C25D: PROCESSES FOR THE ELECTROLYTIC OR ELECTROPHORETIC PRODUCTION OF COATINGS; ELECTROFORMING; APPARATUS THEREFOR
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES

## Abstract

Fabrication of a high sensitivity potentiometric biosensor is described. The present inventors have developed and characterized a novel amplification platform using a gold nanoparticle (GNPs) electrodeposition method. The synthesized GNP sizes were found to be dependent of HAuCl4 concentration, media acid, scan cycles and scan rate. A systematic investigation into the adsorption of different sizes of proteins from aqueous electrolyte solution onto the electrodeposited GNPs surface by the potentiometric method was performed. Results suggest that the size of different proteins affect how they bond to different sizes of GNPs. This GNPs-based biosensor can retain the native-like structure of proteins, and successfully detect proteins at a high sensitivity level. The resulting glucose and immune biosensors also exhibit low detection limit and wide linear range. This improvement to potentiometric devices enables them to serve as highly sensitive detectors for biomolecules and provides a model that can be used to predict protein bonding on nanoparticles.

## Claims

### Claim 1

A method for making a potentiometric biosensor including a specific protein adsorbed onto gold nanoparticles, the method comprising:
a) electrodepositing gold onto an electrode to produce a gold nanoparticle modified electrode, wherein the electrodepositing uses electrodeposition parameters selected to deposit gold nanoparticles on the electrode such that a mean size of the deposited gold nanoparticles corresponds to a size promoting, relative to other mean sizes of deposited gold nanoparticles produced by other combinations of electrodeposition parameters, adsorption of the specific protein onto the gold nanoparticles; and
b) adsorbing the specific protein onto the gold nanoparticle electrode to produce the potentiometric biosensor,
wherein the specific protein selected from a group consisting of (A) Bovine serum albumin (BSA), (B) glucose oxidase (GOx), and (C) Casein, and
wherein if the specific protein is BSA, then the mean size of the deposited gold nanoparticles is 5 nm±2 nm, if the specific protein is GOx, then the mean size of the deposited gold nanoparticles is 14 nm±2 nm, and if the specific protein is Casein, then the mean size of the deposited gold nanoparticles is 40 nm±2 nm.

### Claim 2 (depends on 1)

The method of claim 1 wherein the act of electrodepositing is performed using cyclic voltemmetry electrodeposition.

### Claim 3 (depends on 2)

The method of claim 2 wherein at least one of the electrodepostion parameters is a voltage scan rate.

### Claim 4 (depends on 2)

The method of claim 2 wherein at least one of the electrodepostion parameters is a maximum number of scan cycles.

### Claim 5 (depends on 1)

The method of claim 1 wherein the electrodepositing uses electrodeposition parameters selected to deposit gold nanoparticles on the electrode such that a mean size of the deposited gold nanoparticles corresponds to a size optimizing, relative to other mean sizes of deposited gold nanoparticles produced by other combinations of electrodeposition parameters, adsorption of the specific protein onto the gold nanoparticles.

### Claim 6

A method for making a potentiometric biosensor including a specific protein adsorbed onto gold nanoparticles, the method comprising:
a) electrodepositing gold onto an electrode to produce a gold nanoparticle modified electrode, wherein the electrodepositing uses electrodeposition parameters selected to deposit gold nanoparticles on the electrode such that a mean size of the deposited gold nanoparticles corresponds to a size promoting, relative to other mean sizes of deposited gold nanoparticles produced by other combinations of electrodeposition parameters, adsorption of the specific protein onto the gold nanoparticles; and
b) adsorbing the specific protein onto the gold nanoparticle electrode to produce the potentiometric biosensor,
wherein the act of electrodepositing is performed using cyclic voltemmetry electrodeposition, and
wherein at least one of the electrodepostion parameters is acidic solution concentration.

### Claim 7

A potentiometric biosensor comprising:
a) an electrode;
b) gold nanoparticles coating a surface of the electrode; and
c) a specific protein adsorbed onto the gold nanoparticles,
wherein a mean size of the gold nanoparticles is one that promoted, relative to other mean sizes of deposited gold nanoparticles produced by other combinations of electrodeposition parameters, the adsorption of the specific proteins,
wherein the specific protein selected from a group consisting of (A) Bovine serum albumin (BSA), (B) glucose oxidase (GOx), and (C) Casein, and
wherein if the specific protein is BSA, then the mean size of the deposited gold nanoparticles is 5 nm±2 nm, if the specific protein is GOx, then the mean size of the deposited gold nanoparticles is 14 nm±2 nm, and if the specific protein is Casein, then the mean size of the deposited gold nanoparticles is 40 nm±2 nm.

### Claim 8 (depends on 7)

The potentiometric biosensor of claim 7 wherein the specific protein remains active after its adsorption onto the gold nanoparticles.

### Claim 9 (depends on 7)

The potentiometric biosensor of claim 7 wherein the specific protein is not denatured by its adsorption onto the gold nanoparticles.

### Claim 10 (depends on 7)

The potentiometric biosensor of claim 7 wherein a mean size of the gold nanoparticles is one that optimized, relative to other mean sizes of deposited gold nanoparticles produced by other combinations of electrodeposition parameters, the adsorption of the specific proteins.