# US 10,370,684 B2 · Treatment method of saccharide solution

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- Number: US 10,370,684 B2
- Document ID: US10370684B2
- Status: Granted
- Kind: B2
- Application number: 15/264,017
- Filed: 2016-09-13
- Published: 2016-12-29
- Granted: 2019-08-06
- Earliest claimed: 2014-03-13
- Assignees: Mitsubishi Chemical Corporation
- Applicants: Mitsubishi Chemical Corporation
- Inventors: Kiminori Kawakami; Yasuyo Saito; Takanao Matsumoto; Ryusuke Aoyama; Shigeki Nitta; Masaru Utsunomiya
- Web page: https://patentgrep.com/patent/US10370684B2

## CPC

- C12P 7/46: Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
- C12P 7/06: Ethanol, i.e. non-beverage
- C13K 1/02: obtained by saccharification of cellulosic materials (manufacture of fodder A23K10/32)
- C13K 1/04: Purifying
- C13K 11/00: Fructose (separation from invert sugar C13K3/00)
- C13K 13/00: Sugars not otherwise provided for in this class
- C13K 13/002: {Xylose}
- Y02E 50/10: Biofuels, e.g. bio-diesel
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C13K: SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES (chemically synthesised sugars or sugar derivatives C07H; polysaccharides, e.g. starch, derivatives thereof C08B; malt C12C; fermentation or enzyme-using processes for preparing compounds containing saccharide radicals C12P19/00; production of sucrose C13B)
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

The invention relates to a method for treating a solution containing a saccharide comprising a heat treatment step of heating the saccharide solution at a temperature of 100° C. or higher and 180° C. or lower and a reduction step of reacting a reducing agent with the saccharide solution heated in the heat treatment step, a method for producing a treated saccharide solution using the treatment method, a treated saccharide solution obtained through treatment by the treatment method, a method for producing an organic compound comprising an organic-matter production step of obtaining an organic compound by causing a microorganism capable of producing organic matter to act on an organic raw material containing the treated saccharide solution and a method for culturing a microorganism using the treated saccharide solution as a carbon source.

## Claims

### Claim 1

A method for treating a saccharide solution, comprising:
heating the saccharide solution at a temperature of 100° C. to 180° C., to obtain a heated saccharide solution; and
reacting a reducing agent with the heated saccharide solution.

### Claim 2 (depends on 1)

The method according to claim 1,
wherein the saccharide solution comprises a monosaccharide, a disaccharide, or both.

### Claim 3 (depends on 1)

The method according to claim 1, wherein a pH of the saccharide solution during the reacting is 2 to 8.

### Claim 4 (depends on 1)

The method according to claim 1, wherein a temperature of the saccharide solution during the reacting is 20° C. to 70° C.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the reducing agent is at least one compound selected from the group consisting of a sulfurous acid compound, a hyposulfurous acid compound, and a thiosulfuric acid compound.

### Claim 6 (depends on 1)

The method according to claim 1, wherein an amount of the reducing agent used is 0.05 mass % to 2.0 mass % based on a mass of the saccharide contained in the saccharide solution.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the heating of the saccharide solution is from one minute to 20 hours.

### Claim 8 (depends on 1)

A method for producing an organic compound, comprising:
performing the method of claim 1 to obtain a reduced saccharide solution; and
contacting a microorganism capable of producing organic matter with an organic raw material comprising the reduced saccharide solution.

### Claim 9

A method for producing an organic compound, comprising:
heating a saccharide solution at a temperature of 100° C. to 180° C., to obtain a heated saccharide solution;
reacting a reducing agent with the heated saccharide solution, to obtain a treated saccharide solution; and
culturing a microorganism capable of producing organic matter in a medium comprising the treated saccharide solution as a carbon source.

### Claim 10 (depends on 1)

The method of claim 1,
wherein the reducing agent is an ionic compound,
the ionic compound comprises: an anion containing one or more kinds of sulfur having an oxidation number selected from the group consisting of −2, +2, +3 and +4; and ammonium ion, and
the reducing agent reduces a carbonyl compound except for a saccharide contained in the saccharide solution.
