# US 10,370,676 B2 · Genes related to salt or drought stress resistances and transformed plants with the same

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- Number: US 10,370,676 B2
- Document ID: US10370676B2
- Status: Granted
- Kind: B2
- Application number: 14/327,905
- Filed: 2014-07-10
- Published: 2015-04-30
- Granted: 2019-08-06
- Earliest claimed: 2013-10-24
- Assignees: Industry-Academic Cooperation Foundation, Yonsei University
- Applicants: Industry-Academic Cooperation Foundation, Yonsei University
- Inventors: Woo Taek Kim; Jae Hwan Hwang; Dong Hye Seo
- Web page: https://patentgrep.com/patent/US10370676B2

## CPC

- C12N 15/8273: {for drought, cold, salt resistance}
- C07K 14/415: from plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention relates to a composition for improving a tolerance to a salt stress or a drought stress of a plant, and a method for preparing transformed plant exhibiting improved tolerance to salt stress or drought stress. The nucleotide sequences of the present invention are involved in salt or drought stress-tolerance, therefore may be effectively used for cultivating the plants with novel functional features which are less affected by climates and environments of the cultivated areas.

## Claims

### Claim 1

A method for preparing transformed Arabidopsis thaliana exhibiting improved tolerance to a salt stress, the method comprising:
(a) introducing a transformation vector into the AtSRG1 gene, wherein the transformation vector inhibits an expression of a nucleotide sequence encoding the amino acid sequence consisting of SEQ ID NO:3 into a Arabidopsis thaliana cell;
(b) obtaining the transformed Arabidopsis thaliana exhibiting improved salt stress tolerance from the Arabidopsis thaliana cell of (a); and
(c) growing the transformed Arabidopsis thaliana under a salt stress condition.

### Claim 2

A method for improving a tolerance to a salt stress of Arabidopsis thaliana, the method comprising:
(a) introducing a transformation vector into the AtSRG1 gene, wherein the transformation vector inhibits an expression of a nucleotide sequence encoding the amino acid sequence consisting of SEQ ID NO:3 into a cell of the Arabidopsis thaliana;
(b) obtaining a transgenic Arabidopsis thaliana exhibiting improved tolerance to a salt stress from the cell of the Arabidopsis thaliana; and
(c) growing the transgenic Arabidopsis thaliana under a salt stress condition.

### Claim 3 (depends on 2)

The method according to claim 2, wherein the nucleotide sequence encoding the amino acid sequence of SEQ ID NO:3 is the nucleotide sequence of SEQ ID NO:1.

### Claim 4 (depends on 2)

The method according to claim 2, wherein the transformation vector is T-DNA.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the nucleotide sequence encoding the amino acid sequence of SEQ ID NO:3 is the nucleotide sequence of SEQ ID NO:1.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the transformation vector is T-DNA.
