# US 10,370,674 B2 · Generation of transgenic canola with low or no saturated fatty acids

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,370,674 B2
- Document ID: US10370674B2
- Status: Granted
- Kind: B2
- Application number: 15/398,481
- Filed: 2017-01-04
- Published: 2017-04-27
- Granted: 2019-08-06
- Earliest claimed: 2004-10-08
- Assignees: Dow AgroSciences LLC
- Applicants: Dow AgroSciences LLC
- Inventors: Daniel J. Gachotte; Ann Owens Merlo; Mark A. Thompson; Terence A. Walsh; Beth Rubin Wilson; Mary Welter
- Web page: https://patentgrep.com/patent/US10370674B2

## CPC

- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 9/0083: {Miscellaneous (1.14.99)}
- C12Y 114/00: Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y 114/99: Miscellaneous (1.14.99)
- C12N 5/14: Plant cells
- C12Y 114/19001: Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

Compositions and methods include genetically encoding and expressing a novel delta-9 desaturase in plant cells. In some embodiments, methods of expressing nucleic acids in a plant cell to take advantage of the delta-9 desaturase enzyme's activity, such that the percent composition of saturated fatty acids in plant seeds is decreased and there is a concomitant increase in Δ9 fatty acids. In other embodiments, amino acid sequences have delta-9 desaturase activity. Methods can involve expression of delta-9 desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of mono unsaturated fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

## Claims

### Claim 1

A method for decreasing the amount of saturated fatty acids in oil from a plant, the method comprising:
transforming plant material with a nucleic acid molecule comprising a heterologous gene regulatory element operably linked to a polynucleotide encoding a delta-9 desaturase enzyme comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:1,
wherein the plant material also comprises an extraplastidial desaturase selected from the group consisting of LnD9DS desaturase, HzD9DS desaturase, and MgD9DS desaturase;
culturing the transformed plant material to obtain a plant; and
extracting oil from the plant.

### Claim 2 (depends on 1)

The method of claim 1, wherein the plant material is a seed.

### Claim 3 (depends on 1)

The method of claim 1, wherein the plant is selected from a genus selected from the group consisting of Arabidopsis, Borago, Canola, Ricinus, Theobroma, Zea, Gossypium, Crambe, Cuphea, Linum, Lesquerella, Limnanthes, Linola, Tropaeolum, Oenothera, Olea, Elaeis, Arachis, rapeseed, Carthamus, Glycine, Soja, Hehanthus, Nicotiana, Vernonia, Triticum, Hordeum, Oryza, Avena, Sorghum, Secale, and the other members of the Gramineae.