# US 10,370,672 B2 · Means and methods for regulating secondary metabolite production in plants

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- Number: US 10,370,672 B2
- Document ID: US10370672B2
- Status: Granted
- Kind: B2
- Application number: 15/119,681
- Filed: 2015-02-18
- Published: 2017-01-12
- Granted: 2019-08-06
- Earliest claimed: 2014-02-18
- Assignees: Vib Xvzw; Universiteit Gent; Universiteit Leiden
- Applicants: Vib Vzw; Universiteit Gent; Universiteit Leiden
- Inventors: Alain Goossens; Jan Mertens; Alex Van Moerkercke; Jacob Pollier; Johan Memelink
- Web page: https://patentgrep.com/patent/US10370672B2

## CPC

- C12N 15/8243: {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
- C07K 14/415: from plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

This disclosure relates to the field of secondary metabolite production in plants. More specifically, the disclosure relates to chimeric genes and their use in the regulation of biosynthesis and/or production of secondary metabolites in plants and plant-derived cell cultures.

## Claims

### Claim 1

A chimeric gene comprising the following operably-linked nucleic acid sequences:
one or more control sequences able to drive expression of a nucleic acid sequence in a plant cell; and a nucleic acid sequence encoding a BHLH protein of subfamily IVa comprising a bHLH domain wherein the nucleic acid sequence is SEQ ID NO: 15.

### Claim 2 (depends on 1)

A transgenic plant comprising:
the chimeric gene of claim 1.

### Claim 3 (depends on 1)

A plant cell comprising:
the chimeric gene of claim 1.

### Claim 4 (depends on 1)

A vector comprising the chimeric gene of claim 1.

### Claim 5 (depends on 1)

The chimeric gene of claim 1, further comprising:
a transcript termination nucleic acid sequence operably-linked thereto.