# US 10,370,640 B2 · Self-aligned fibrous scaffolds for automechanoinduction of cell cultures

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- Number: US 10,370,640 B2
- Document ID: US10370640B2
- Status: Granted
- Kind: B2
- Application number: 15/644,954
- Filed: 2017-07-10
- Published: 2018-01-18
- Granted: 2019-08-06
- Earliest claimed: 2016-07-12
- Assignees: Tallinn University of Technology
- Applicants: Tallinn University of Technology
- Inventors: Irina Hussainova; Michael Gasik; Roman Ivanov
- Web page: https://patentgrep.com/patent/US10370640B2

## CPC

- C12N 5/0619: {Neurons}
- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- C12N 5/0645: {Macrophages, e.g. Kuepfer cells in the liver; Monocytes}
- C12N 5/0667: {Adipose-derived stem cells [ADSC]; Adipose stromal stem cells}
- C12N 5/0693: {Tumour cells; Cancer cells}
- G01N 33/5005: {involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025)}
- C12N 2503/00: Use of cells in diagnostics
- C12N 2506/1346: from mesenchymal stem cells
- C12N 2513/00: 3D culture
- C12N 2527/00: Culture process characterised by the use of mechanical forces, e.g. strain, vibration
- C12N 2533/00: Supports or coatings for cell culture, characterised by material
- C12N 2533/10: Mineral substrates
- C12N 2533/30: Synthetic polymers
- C12N 2535/10: Patterned coating
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Self-aligned fibrous scaffolds are disclosed. The scaffolds are capable of automechanoinduction of cell cultures and methods to induce authomechanoinduction in cancer cells and stem cells are disclosed as well.

## Claims

### Claim 1

A biocompatible scaffold for multi-dimensional cultivation of cells, said scaffolds capable of automechanoinduction and comprising:
aligned fibers oriented to form a scaffold with open porosity for the cultured cells, wherein the fibers have a diameter of 5-100 nm, and an aspect ratio more than 10000:1,
said fibers being made of non-cytotoxic, non-carcinogenic inorganic metal oxide, and
said fibers being self-aligned into the scaffold during its manufacturing process.

### Claim 2 (depends on 1)

The scaffold according to claim 1, wherein the open porosity is in a range of 70-99%.

### Claim 3 (depends on 2)

The scaffold according to claim 2, wherein the open porosity is 85-95%.

### Claim 4 (depends on 1)

The scaffold according to claim 1, wherein the fibers have a length of 10-20 mm.

### Claim 5 (depends on 1)

The scaffold according to claim 1, wherein the diameter of the fibers is 20-50 nm.

### Claim 6 (depends on 1)

The scaffold according to claim 1, wherein the fibers have aspect ratio more than 20000:1.

### Claim 7 (depends on 1)

The scaffold according to claim 1, wherein the fibers are coated with a bioinert material.

### Claim 8 (depends on 7)

The scaffold according to claim 7, wherein the bioinert material is non-functionalized graphene.

### Claim 9 (depends on 1)

The scaffold according to claim 1, wherein the metal oxide is selected from the group consisting of silica, titania, zirconia and alumina.

### Claim 10 (depends on 9)

The scaffold of according to claim 9, wherein the fibers are of aluminum oxide.

### Claim 11 (depends on 1)

The scaffold of claim 1, wherein the scaffold is for cultivation of stem cells and the scaffold is capable of inducing automechanoinduction of neural differentiation of the stem cells.

### Claim 12 (depends on 1)

The scaffold of claim 1, wherein the scaffold is for cultivation of cancer cells and the scaffold is capable of inducing automechanoinduction of gene expressions in the cancer cells to make them suitable as tumor models.

### Claim 13 (depends on 1)

A method to induce automechanoinduction of neural differentiation of stem cells by cultivating the stem cells on a scaffold of claim 1.

### Claim 14 (depends on 1)

A method to induce automechanoinduction of gene expressions in cancer cells by cultivating the cancer cells on a scaffold of claim 1.