# US 10,370,632 B2 · Facility for culturing pluripotent stem cells

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- Number: US 10,370,632 B2
- Document ID: US10370632B2
- Status: Granted
- Kind: B2
- Application number: 15/035,879
- Filed: 2014-07-11
- Published: 2016-09-22
- Granted: 2019-08-06
- Earliest claimed: 2013-11-12
- Assignees: Kajima Corporation
- Applicants: Tokyo Electron Limited; Kajima Corporation
- Inventors: Toshimitsu Fuji; Shigenori Ozaki; Yoshio Kinoshita; Hajime Fujinaga; Ryoji Shiina; Atsushi Nishida
- Web page: https://patentgrep.com/patent/US10370632B2

## CPC

- C12M 23/58: {Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00)}
- C12M 35/00: {Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion (machines for extracting juice from animal or plant tissue by electroplasmolysis A23N1/006, processes employing electric or wave energy B01J19/08; treatment of microorganisms or enzymes with electrical or wave energy C12N13/00; methods for cell fusion C12N15/02; introduction of foreign genetic material C12N15/87)}
- C12M 21/08: {for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00)}
- C12M 37/00: {Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00)}
- C12M 41/46: {of cellular or enzymatic activity or functionality, e.g. cell viability}
- C12N 5/0607: {Non-embryonic pluripotent stem cells, e.g. MASC (induced pluripotent stem cells C12N5/0696)}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

In order to culture the pluripotent stem cell which can be variously differentiated, while preventing the cross contamination between the different cells and securing the safety of the products; extending a main transport path 31 outwardly from a stem cell conditioning area 20, the stem cell conditioning area 20 including a treatment room 21 for inducing pluripotent stem cell from a somatic cell or an egg cell, or a treatment room 21 for receiving and conditioning pluripotent stem cell induced in other facilities; branching at least one branched transport path 32 from the main transport path 31; and arranging along each branched transport path 32, a cell culture area 40 including culture rooms 41 to 44 for culturing the stem cell and an analysis room 45 for analyzing the cultured cell, respectively. Preferably, the stem cell conditioning area 20, the cell culture area 40 and a transport area 30 including the main transport path 31 and the branched transport path 32 are provided with respective operator gates 22, (47+48), 33 individually, so as to prohibit coming and going of operator among the areas 20, 40, 30.

## Claims

### Claim 1

A facility for culturing pluripotent stem cell comprising:
a stem cell conditioning area including a treatment room for receiving a somatic cell or an egg cell from outside and inducing pluripotent stem cell from the somatic cell or the egg cell, or a treatment room for receiving and conditioning pluripotent stem cell induced in other facilities from outside;
a transport area including a main transport path extending outwardly from the treatment room and a plurality of branched transport paths from the main transport path; and
a plurality of cell culture areas including a culture room for culturing the stem cell as differentiating into a particular cell or without differentiation and an analysis room for analyzing the cultured cells, and being arranged along each branched transport path respectively,
wherein the treatment room in the stem cell conditioning area is connected with the main transport path via a transfer-opening, and the culture room and the analysis room in each cell culture area are connected with the branched transport path, respectively, via a transfer-opening, and
the stem cell conditioning area, each of the cell culture area, and the transport area are provided with respective operator gates to outside of all areas individually, and no operator gate is provided between the stem cell conditioning area and the transport area, no operator gate is provided between the transport area and each of the cell culture area, and no operator gate is provided between each of the cell culture area.

### Claim 2 (depends on 1)

The facility for culturing pluripotent stem cell according to claim 1, wherein a storage room for storing the cells cultured in each cell culture area is provided along the main transport path in the transport area, the storage room being connected with the main transport path via a transfer-opening.

### Claim 3 (depends on 2)

The facility for culturing pluripotent stem cell according to claim 2, wherein the storage room leads to a delivery preparation room via a transfer-opening.

### Claim 4 (depends on 1)

The facility for culturing pluripotent stem cell according to claim 1, wherein along at least one branched transport path, the culture area is arranged that includes the culture room for culturing the undifferentiated pluripotent stem cell and the analysis room for analyzing it; and along other branched transport path, the culture area is arranged that includes the culture room for culturing a particular cell differentiated from the stern cell and the analysis room for analyzing said particular cell.

### Claim 5 (depends on 2)

The facility for culturing pluripotent stem cell according to claim 2, wherein along at least one branched transport path, the culture area is arranged that includes the culture room for culturing the undifferentiated pluripotent stem cell and the analysis room for analyzing it; and along other branched transport path, the culture area is arranged that includes the culture room for culturing a particular cell differentiated from the stem cell and the analysis room for analyzing said particular cell.

### Claim 6 (depends on 3)

The facility for culturing pluripotent stem cell according to claim 3, wherein along at least one branched transport path, the culture area is arranged that includes the culture room for culturing the undifferentiated pluripotent stem cell and the analysis room for analyzing it; and along other branched transport path, the culture area is arranged that includes the culture room for culturing a particular cell differentiated from the stem cell and the analysis room for analyzing said particular cell.

### Claim 7 (depends on 1)

The facility for culturing pluripotent stem cell according to claim 1, wherein along each branched transport paths, the culture areas are arranged that includes the culture room for culturing the undifferentiated pluripotent stem cell; and connecting to each branched transport paths, the analysis room is arranged for analyzing the cultured cells jointly.

### Claim 8 (depends on 2)

The facility for culturing pluripotent stem cell according to claim 2, wherein along each branched transport paths, the culture areas are arranged that includes the culture room for culturing the undifferentiated pluripotent stem cell; and connecting to each branched transport paths, the analysis room is arranged for analyzing the cultured cells jointly.

### Claim 9 (depends on 3)

The facility for culturing pluripotent stem cell according to claim 3, wherein along each branched transport paths, the culture areas are arranged that includes the culture room for culturing the undifferentiated pluripotent stem cell; and connecting to each branched transport paths, the analysis room is arranged for analyzing the cultured cells jointly.