# US 10,370,155 B2 · Material and packaging for yeast storage

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- Number: US 10,370,155 B2
- Document ID: US10370155B2
- Status: Granted
- Kind: B2
- Application number: 14/363,838
- Filed: 2012-12-13
- Published: 2014-12-18
- Granted: 2019-08-06
- Earliest claimed: 2011-12-14
- Assignees: Lesaffre Et Compagnie
- Applicants: Lesaffre Et Compagnie
- Inventors: Sandrine Dobel; Anthony Bernard Malaquin
- Web page: https://patentgrep.com/patent/US10370155B2

## CPC

- B65D 33/01: Ventilation or drainage of bags
- B32B 3/266: {characterised by an apertured layer, the apertures going through the whole thickness of the layer, e.g. expanded metal, perforated layer, slit layer regular cells B32B3/12}
- B32B 7/02: Physical, chemical or physicochemical properties
- B32B 27/08: of synthetic resin
- B32B 27/306: {comprising vinyl acetate or vinyl alcohol (co)polymers}
- B32B 27/34: comprising polyamides
- B32B 27/36: comprising polyesters
- B65D 85/72: for edible or potable liquids, semiliquids, or plastic or pasty materials
- C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00)
- C12N 1/18: Baker's yeast; Brewer's yeast
- B32B 2250/24: All layers being polymeric
- B32B 2250/40: Symmetrical or sandwich layers, e.g. ABA, ABCBA, ABCCBA
- B32B 2270/00: Resin or rubber layer containing a blend of at least two different polymers
- B32B 2307/724: Permeability to gases, adsorption
- B32B 2307/7244: Oxygen barrier
- B32B 2439/40: Closed containers
- B32B 2439/70: Food packaging
- B65D: CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention relates to the use of a three-layer plastic film that is permeable to carbon dioxide but slightly impermeable to oxygen, as packing for liquid or semi-liquid products which produce gas. When the plastic film is reinforced with a uniformly perforated second plastic film, the resulting material is suitable for the manufacture of packaging for the storage of liquid or semi-liquid products containing yeasts, such as cream yeast.

## Claims

### Claim 1

Method for the storage of a liquid or semi-liquid yeast or leaven, said method comprising a step of:
storing the liquid or semi-liquid yeast or leaven in a packaging,
wherein:
the packaging comprises a container composed of two plastic films, wherein the first plastic film is a three-layer plastic film with a B-A-B′ structure and the second plastic film is uniformly perforated;
the first plastic film defines the internal volume of the container; and
the container is in the form of a pouch having a total internal volume of between 1 liter and 1000 liters;
and wherein:
layer A of the three-layer plastic film consists of an ethylene-vinyl acetate (EVA) with a high vinyl acetate content, as percentage by weight, of between 18% and 42%, and
each of layers B and B′ of the three-layer plastic film consists of an ethylene-vinyl acetate (EVA) having a vinyl acetate content, as percentage by weight, which is lower than that of the ethylene-vinyl acetate of layer A,
the permeability of the three-layer plastic film to carbon dioxide, measured according to ISO standard 15105-2:2003 annex B, is greater than or equal to 80 l/m2·24 h at delta P=1 bar,
the three-layer plastic film has a total thickness of from 20 to 50 microns, and
the permeability of the three-layer plastic film to oxygen (O2), measured according to ISO standard 1510-2:2003 annex B, is less than or equal to 30 l/m2·24 h at delta P=1 bar.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the liquid or semi-liquid yeast is a cream yeast.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the permeability of the three-layer plastic film to carbon dioxide, measured according to ISO standard 15105-2:2003 annex B, is greater than or equal to 90 l/m2·24 h at delta P=1 bar.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the layers B and B′ of the three-layer plastic film are identical.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the layers B and B′ of the three-layer plastic film are different.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the container is in the form of a pouch having a total internal volume of between 10 liters and 200 liters.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the container is in the form of a pouch having a total internal volume of between 1 liter and 50 liters.

### Claim 8 (depends on 1)

The method according to claim 1, wherein the second plastic film is composed of oriented polyamide (OPA) and polyethylene (PE) or of polyethylene terephthalate (PET) and polyethylene (PE).

### Claim 9 (depends on 1)

The method according to claim 1, wherein the pouch comprises a base and a cap.

### Claim 10 (depends on 1)

The method according to claim 1, wherein the packaging is in the form of a bag-in-box and also comprises a cardboard box comprising an opening.

### Claim 11 (depends on 1)

The method according to claim 1 further comprising a step of:
storing, at a temperature of between 0 and 6° C. and in a relative humidity of between 50% and 100%, said packaged ingredient until use.

### Claim 12 (depends on 2)

The method according to claim 2, wherein the cream yeast is a baker's cream yeast.