# US 10,369,626 B2 · Process for the preparation of bio-organic coated gold and silver nanoparticles using blue light

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- Number: US 10,369,626 B2
- Document ID: US10369626B2
- Status: Granted
- Kind: B2
- Application number: 15/561,625
- Filed: 2016-03-30
- Published: 2018-03-15
- Granted: 2019-08-06
- Earliest claimed: 2015-03-30
- Assignees: Council of Scientific & Industrail Research
- Applicants: Council of Scientific & Industrial Research
- Inventors: Ashish; Yogendra Singh Rathore; Nagesh Peddada; Kunzes Dolma; Renu Garg
- Web page: https://patentgrep.com/patent/US10369626B2

## CPC

- B22F 9/24: starting from liquid metal compounds, e.g. solutions
- A61K 47/62: the modifying agent being a protein, peptide or polyamino acid
- A61K 47/6923: {the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb}
- A61P 5/48: of the pancreatic hormones
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- B22F 1/054: Nanosized particles
- B22F 1/102: Metallic powder coated with organic material
- B22F 1/148: Agglomerating
- G01N 21/255: {Details, e.g. use of specially adapted sources, lighting or optical systems}
- G01N 21/78: producing a change of colour
- B22F 2301/255: Silver or gold
- B22F 2999/00: Aspects linked to processes or compositions used in powder metallurgy
- B22F: WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER (making alloys by powder metallurgy C22C); APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to a process which uses blue light to form bio-organic coated silver and gold nanoparticles for various applications. The present invention particularly relates to increasing the rates of reaction and widening the scope of tethering a range of biomolecules on the metal nanoparticles. The examples demonstrate that the biomolecules immobilized on the nanoparticles still retain many aspects of their structural elements which allow them to bind to their native binding partners and/or retain structural epitopes which allow them to be identified by antibodies specific to them, thus opening up scope of using such coated nanoparticles for functionalized and specific applications.

## Claims

### Claim 1

A process for the preparation of bio-organic coated gold nanoparticle or bio-organic coated silver nanoparticles using blue light, comprising the steps of:
(a) preparing a solution of AgNO3 or HAuCl4 in the range of 60-300 μM, optionally in a buffer having pH of 5 to 8 or in water having 0.1 to 300 mM NaCl;
(b) adding pure biomolecules to the solution prepared in step (a), wherein the molar ratio of AgNO3 to biomolecule is 50:1 or the molar ratio of HAuCl4 to biomolecule is 300:1, wherein the biomolecules are selected from the group consisting of peptides, antibodies, proteins, aptamers and oligonucleotides;
(c) exposing the mixture of step (b) to blue light having a wattage in the range of 40 W to 200 W for 0.1 to 60 hours at a temperature of 30° C. to obtain the bio-organic coated gold nanoparticle or bio-organic coated silver nanoparticles.

### Claim 2 (depends on 1)

The process as claimed in claim 1, wherein the buffer is selected from HEPES, tris, phosphate or trisodium citrate buffer.

### Claim 3 (depends on 1)

The process as claimed in claim 1, wherein the wavelength of blue light is in the range of 350 nm to 450 nm.

### Claim 4 (depends on 1)

The process as claimed in claim 1, wherein the biomolecule is gelsolin.

### Claim 5 (depends on 1)

The process as claimed in claim 1, wherein the biomolecule is insulin.

### Claim 6 (depends on 1)

The process as claimed in claim 1, wherein the formation of bio-organic coated gold nanoparticles or bio-organic coated silver nanoparticles is monitored by observing color change of the mixture or by measuring the absorbance of the mixture at single wavelength of 420 nm for bio-organic coated silver nanoparticles or at single wavelength of 550 nm for bio-organic coated gold nanoparticles.

### Claim 7 (depends on 6)

The process as claimed in claim 6, wherein the color change of the mixture from colorless to pale yellow to dark yellow indicates the formation of bio-organic coated silver nanoparticles.

### Claim 8 (depends on 6)

The process as claimed in claim 6, wherein the color change of the reaction mixture from colorless to light pink to purple indicates the formation of bio-organic coated gold nanoparticles.