# US 10,369,240 B2 · Targeted enzymatic degradation of quorum-sensing peptides > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,369,240 B2 - Document ID: US10369240B2 - Status: Granted - Kind: B2 - Application number: 15/802,817 - Filed: 2017-11-03 - Published: 2018-03-08 - Granted: 2019-08-06 - Earliest claimed: 2012-03-20 - Assignees: KCI Licensing, Inc. - Applicants: KCI Licensing, Inc. - Inventors: Rodolfo M. Alarcon; Amy K. McNulty - Web page: https://patentgrep.com/patent/US10369240B2 ## CPC - A61L 2/16: using chemical substances - A61K 38/48: acting on peptide bonds (3.4) - A61K 39/395: Antibodies (agglutinins A61K38/36 {; as drug carriers A61K47/50}); Immunoglobulins; Immune serum, e.g. antilymphocytic serum - A61K 39/40: bacterial - A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca - A61L 29/16: Biologically active materials, e.g. therapeutic substances {(A61L29/048 takes precedence)} - C07K 14/001: {by chemical synthesis} - C07K 16/12: against material from bacteria - A61L 2300/404: Biocides, antimicrobial agents, antiseptic agents - Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Methods and compositions for the treatment of biofilms and/or the inhibition of biofilm formation. In one embodiment, a biofilm is treated and/or biofilm formation is inhibited by a method comprising contacting a biofilm or a surface with a bifunctional ligand comprising a quorum-sensing-peptide-binding region and a protease-binding region, whereby the biofilm is treated and/or biofilm formation on the surface is inhibited. ## Claims ### Claim 1 A dressing for treating a wound, comprising: a distribution manifold comprising collagen; and a bifunctional ligand disposed on a surface of the distribution manifold and comprising a quorum-sensing-molecule-binding region chemically linked to a protease-binding region. ### Claim 2 (depends on 1) The dressing of claim 1, wherein the distribution manifold further comprises oxidized regenerated cellulose. ### Claim 3 (depends on 1) The dressing of claim 1, further comprising a sealing member adapted to be positioned over the distribution manifold and the wound to create a sealed environment. ### Claim 4 (depends on 1) The dressing of claim 1, further comprising: a sealing member adapted to be positioned over the distribution manifold and the wound to create a sealed environment; and a reduced-pressure source adapted to be in fluid communication with the sealed environment. ### Claim 5 (depends on 1) The dressing of claim 1, wherein the quorum-sensing-molecule-binding region is adapted to bind at least one of an acylated homoserine lactone (AHL), an autoinducing peptide (AlP), AI-2 (a furanosyl borate diester), a γ-butyrolactone, and a competence stimulating peptide (CSP). ### Claim 6 (depends on 1) The dressing of claim 1, wherein the distribution manifold further comprises gelatin. ### Claim 7 (depends on 1) The dressing of claim 1, wherein the protease-binding region is adapted to bind a lactonase. ### Claim 8 (depends on 1) The dressing of claim 1, further comprising a peptide linker between the quorum-sensing-molecule-binding region and the protease-binding region. ### Claim 9 A system for treating a tissue site, comprising: a dressing comprising a bioresorbable material and a bifunctional ligand comprising a quorum-sensing-molecule-binding region chemically linked to a protease-binding region; a sealing member adapted to be positioned over the dressing and the tissue site to create a sealed environment; and a reduced-pressure source adapted to be in fluid communication with the sealed environment. ### Claim 10 (depends on 9) The system of claim 9, wherein the bioresorbable material comprises collagen. ### Claim 11 (depends on 9) The system of claim 9, wherein the bioresorbable material comprises chitosan. ### Claim 12 (depends on 9) The system of claim 9, wherein the bioresorbable material comprises collagen and oxidized regenerated cellulose. ### Claim 13 (depends on 9) The system of claim 9, wherein the bioresorbable material comprises at least one of polylactic acid and polyglycolic acid. ### Claim 14 (depends on 9) The system of claim 9, wherein the bifunctional ligand further comprises a peptide linker between the quorum-sensing-molecule-binding region and the protease-binding region. ### Claim 15 (depends on 9) The system of claim 9, wherein the dressing further comprises an antibiotic compound. ### Claim 16 A dressing for treating a wound, comprising: a carrier material adapted to be positioned on the wound; and a composition adapted to be applied to the carrier material and comprising: a bifunctional ligand comprising a quorum-sensing-molecule-binding region chemically linked to a protease-binding region, and a therapeutic agent for treating the wound. ### Claim 17 (depends on 16) The dressing of claim 16, wherein the therapeutic agent comprises an antibiotic. ### Claim 18 (depends on 16) The dressing of claim 16, wherein the therapeutic agent comprises a substance for promoting cell growth. ### Claim 19 (depends on 16) The dressing of claim 16, wherein the carrier material comprises a polysaccharide. ### Claim 20 (depends on 16) The dressing of claim 16, wherein the carrier material comprises collagen.