# US 10,369,238 B2 · Method for sterilizing membrane comprising glucose oxidase and associated bio-sensor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,369,238 B2 - Document ID: US10369238B2 - Status: Granted - Kind: B2 - Application number: 15/884,310 - Filed: 2018-01-30 - Published: 2018-06-07 - Granted: 2019-08-06 - Earliest claimed: 2012-05-31 - Assignees: General Electric Company - Applicants: General Electric Company - Inventors: Yida Xu; Rui Chen; Lin Chen - Web page: https://patentgrep.com/patent/US10369238B2 ## CPC - A61L 2/081: Gamma radiation - C12M 41/32: {of substances in solution} - C12Q 1/006: {for glucose} - C12Q 1/26: involving oxidoreductase - A61L 2103/05: Living organisms or biological materials - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract A method for sterilizing a membrane comprising an oxidoreductase enzyme, comprises: irradiating with gamma radiation the membrane comprising an oxidoreductase enzyme soaked in an aqueous buffer solution. Associated biosensors and bioreactors are also described. ## Claims ### Claim 1 A method for sterilizing a membrane comprising an oxidoreductase enzyme, the method comprising: irradiating with gamma radiation the membrane comprising the oxidoreductase enzyme soaked in a phosphate buffer saline (PBS) buffer solution, wherein the PBS buffer consists essentially of sodium phosphate dibasic, sodium acid phosphate, sodium chloride, glycine, N, N′-ethanediybis (N-carboxymethyl) dipotassium, sodium benzoate, and gentamicin sulphate in water. ### Claim 2 (depends on 1) The method of claim 1, wherein the oxidoreductase enzyme is selected from the group consisting of glucose oxidase, lactate oxidase and glutamate oxidase. ### Claim 3 (depends on 2) The method of claim 2, wherein the oxidoreductase enzyme is glucose oxidase. ### Claim 4 (depends on 1) The method of claim 1, wherein the wherein the gamma radiation is conducted at a rate of more than 3 kGy/hour and a dose of at least 25 kGy. ### Claim 5 (depends on 1) The method of claim 1, wherein the aqueous buffer solution is substantially free from organic components. ### Claim 6 A method for sterilizing a membrane comprising glucose oxidase, the method comprising: making a phosphate buffer saline (PBS) solution by dissolving a PBS buffer powder in water, wherein the PBS buffer powder consists essentially of 50-55% wt sodium phosphate dibasic, 10-15% wt sodium acid phosphate, 16-22% wt sodium chloride, less than 10% wt glycine and N, N′-ethanediylbis (N-carboxymethyl) dipotassium, less than 10% wt sodium benzoate, and less than 1% wt gentamicin sulphate, and irradiating with gamma radiation the membrane comprising glucose oxidase soaked in a phosphate buffer saline (PBS) solution, wherein the gamma radiation is carried out in a range of from about 25 kGy to about 40 kGy at rate of about 20 kGy/hour. ### Claim 7 (depends on 1) The method of claim 1, wherein a dose of the gamma radiation is in a range of from about 25 kGy to about 40 kGy. ### Claim 8 (depends on 1) The method of claim 1, wherein a rate of the gamma radiation is about 20 kGy/hour. ### Claim 9 (depends on 1) The method of claim 1, wherein the membrane is in a plastic container. ### Claim 10 (depends on 1) The method of claim 1, wherein the membrane is in a biosensor. ### Claim 11 (depends on 10) The method of claim 10, wherein the biosensor comprises a Pt electrode and an Ag/AgCl electrode. ### Claim 12 (depends on 6) The method of claim 6, wherein the biosensor comprises a Pt electrode and an Ag/AgCl electrode. ### Claim 13 (depends on 1) A biosensor comprising the membrane sterilized using the method of claim 1. ### Claim 14 (depends on 13) The biosensor of claim 13, wherein the membrane comprises glucose oxidase and the sensitivity of the biosensor to glucose is about 80% of the sensitivity of a biosensor comprising a membrane comprising glucose oxidase without sterilization to glucose. ### Claim 15 (depends on 13) The biosensor of claim 13, comprising a Pt electrode and an Ag/AgCl electrode. ### Claim 16 (depends on 13) A bioreactor comprising the biosensor of claim 13. ### Claim 17 A biosensor comprising a sterilized membrane that comprises glucose oxidase, wherein the membrane is sterilized by: irradiating with gamma radiation while the membrane comprising glucose oxidase is soaked in a phosphate buffer saline (PBS) solution, wherein the PBS buffer is made by dissolving a PBS powder in water, wherein the PBS buffer powder consists essentially of 50-55% wt sodium phosphate dibasic, 10-15% wt sodium acid phosphate, 16-22% wt sodium chloride, less than 10% wt glycine and N, N′-ethanediylbis (N-carboxymethyl) dipotassium, less than 10% wt sodium benzoate, and less than 1% wt gentamicin sulphate, and wherein the gamma radiation is carried out in a range of from about 25 kGy to about 40 kGy at rate of about 20 kGy/hour. ### Claim 18 (depends on 17) The biosensor of claim 17, wherein the biosensor comprises a Pt electrode and an Ag/AgCl electrode. ### Claim 19 (depends on 17) The biosensor of claim 17, wherein the sensitivity of the sterilized biosensor to glucose is about 80% of the sensitivity of a biosensor prior to sterilization. ### Claim 20 (depends on 17) A bioreactor comprising the biosensor of claim 17.