# US 10,369,237 B2 · Sterilization and filtration of peptide compositions

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- Number: US 10,369,237 B2
- Document ID: US10369237B2
- Status: Granted
- Kind: B2
- Application number: 15/122,749
- Filed: 2015-03-10
- Published: 2017-07-20
- Granted: 2019-08-06
- Earliest claimed: 2014-03-10
- Assignees: 3-D Matrix, Ltd.
- Applicants: 3-D Matrix, Ltd.
- Inventors: Eun Seok Gil; Karl Patrick Gilbert
- Web page: https://patentgrep.com/patent/US10369237B2

## CPC

- A61L 2/022: Filtration
- A61L 2/04: Heat (radiation A61L2/08)
- A61L 2/07: Steam
- C07K 7/08: having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68)
- A61L 2103/05: Living organisms or biological materials
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Methods and devices for sterilizing viscous peptide compositions which have shear thinning rheological properties at high concentrations.

## Claims

### Claim 1

A method for sterilizing a liquid peptide composition whose sequence comprises a series of repeating units of IEIK as set forth in SEQ ID NO:6 comprising subjecting the liquid peptide composition to autoclave treatment.

### Claim 2 (depends on 1)

The method of claim 1, wherein the method does not involve sterilizing filtration.

### Claim 3

A method for sterilizing a liquid peptide composition whose sequence comprises a series of repeating units of IEIK as set forth in SEQ ID NO:6 comprising subjecting the liquid peptide composition to heat treatment performed at about 121° C. for about 25 minutes.

### Claim 4 (depends on 1)

The method of claim 1, wherein the liquid peptide composition has a concentration within the range of about 0.01% to about 10%.

### Claim 5 (depends on 1)

The method of claim 1, wherein the liquid peptide composition comprises IEIK13 as set forth in SEQ ID NO: 3.

### Claim 6 (depends on 1)

The method of claim 1, wherein the method does not degrade the liquid peptide composition.

### Claim 7 (depends on 3)

The method of claim 3, wherein the liquid peptide composition has a concentration within the range of about 0.01% to about 10%.

### Claim 8 (depends on 3)

The method of claim 3, wherein the liquid peptide composition comprises IEIK13 as set forth in SEQ ID NO: 3.

### Claim 9 (depends on 3)

The method of claim 3, wherein the method does not degrade the liquid peptide composition.