# US 10,369,200 B2 · Platelet activation and growth factor release using electric pulses

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- Number: US 10,369,200 B2
- Document ID: US10369200B2
- Status: Granted
- Kind: B2
- Application number: 15/252,032
- Filed: 2016-08-30
- Published: 2016-12-22
- Granted: 2019-08-06
- Earliest claimed: 2014-01-17
- Assignees: General Electric Company
- Applicants: General Electric Company
- Inventors: Vasile Bogdan Neculaes; Andrew Soliz Torres; Antonio Caiafa; Brian Dun-Lan Lee; Allen Lawrence Garner
- Web page: https://patentgrep.com/patent/US10369200B2

## CPC

- A61K 38/1858: {Platelet-derived growth factor [PDGF]}
- A61K 35/19: Platelets; Megacaryocytes
- A61P 7/04: Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
- C12N 13/00: Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
- G01N 33/48707: {by electrical means (G01N33/49, G01N33/493 take precedence)}
- G01N 33/49: Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Methods and systems for releasing growth factors are disclosed. In certain embodiments, a blood sample is exposed to a sequence of one or more electric pulses to trigger release of a growth factor in the sample. In certain embodiments, the growth factor release is not accompanied by clotting within the blood sample.

## Claims

### Claim 1

A method for releasing growth factors, comprising:
exposing a blood sample to a sequence of one or more electric pulses that trigger release of a growth factor in the blood sample, wherein the growth factor release is not accompanied by clotting within the blood sample.

### Claim 2 (depends on 1)

The method of claim 1, comprising:
collecting the growth factor; and
treating a patient with the growth factor, wherein the blood sample was acquired from the patient.

### Claim 3 (depends on 1)

The method of claim 1, wherein calcium ions are not added to the blood sample prior to exposing the blood sample to the sequence of one or more electric pulses.

### Claim 4 (depends on 1)

The method of claim 1, wherein the method comprises increasing calcium ion concentration and adding an ADP blocking agent to the blood sample prior to exposing the blood sample to the sequence of one or more electric pulses.

### Claim 5 (depends on 4)

The method of claim 4, wherein the ADP blocking chemical comprises apyrase.

### Claim 6 (depends on 1)

The method of claim 1, wherein the one or more electric pulses have an electric field strength between about 0.1 kV/cm and about 350 kV/cm.

### Claim 7 (depends on 1)

The method of claim 1, wherein the one or more electric pulses have pulse durations between about 1 nanosecond and about 100 microseconds.

### Claim 8 (depends on 1)

The method of claim 1, wherein the growth factor comprises platelet derived growth factor.

### Claim 9 (depends on 1)

The method of claim 1, wherein the blood sample is a whole blood sample or a platelet rich plasma.