# US 10,369,089 B2 · Iron oxide nanoparticles and methods of use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,369,089 B2 - Document ID: US10369089B2 - Status: Granted - Kind: B2 - Application number: 15/550,267 - Filed: 2016-02-12 - Published: 2018-02-01 - Granted: 2019-08-06 - Earliest claimed: 2015-02-13 - Assignees: The Trustees of the University of Pennsylvania - Applicants: The Trustees of the University of Pennsylvania - Inventors: Hyun Koo; Lizeng Gao; David Cormode; Pratap Naha - Web page: https://patentgrep.com/patent/US10369089B2 ## CPC - A61K 8/19: containing inorganic ingredients - A01N 59/16: Heavy metals; Compounds thereof - A61K 8/0245: {Specific shapes or structures not provided for by any of the groups of A61K8/0241} - A61K 8/20: Halogens; Compounds thereof - A61K 8/21: Fluorides; Derivatives thereof - A61K 8/22: Peroxides; Oxygen; Ozone - A61K 8/24: Phosphorous; Compounds thereof - A61K 8/66: Enzymes - A61K 8/73: Polysaccharides - A61K 9/14: Particulate form, e.g. powders, {Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles (microspheres A61K9/16; microcapsules A61K9/50; nanocapsules, nanoparticles of the matrix type A61K9/51)} - A61K 9/5161: {Polysaccharides, e.g. alginate, chitosan, cellulose derivatives; Cyclodextrin} - A61K 33/26: Iron; Compounds thereof - A61K 33/40: Peroxides - A61K 47/6923: {the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb} - A61P 1/02: Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis - A61P 13/02: of urine or of the urinary tract, e.g. urine acidifiers - A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like - A61P 19/08: for bone diseases, e.g. rachitism, Paget's disease - A61P 27/16: Otologicals - A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics - A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 - A61Q 11/00: Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses - A61K 9/006: {Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays} - A61K 9/0063: {Periodont} - A61K 2800/413: Nanosized, i.e. having sizes below 100 nm - A61K 2800/624: by macromolecular compounds - A61K 2800/882: Mixing prior to application - A61K 2800/92: Oral administration - Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract The presently disclosed subject matter relates to iron oxide nanoparticle compositions and formulations thereof for: (1) the treatment and elimination of biofilms; (2) the prevention of biofilm formation; (3) biofilm extracellular matrix degradation; (4) the inhibition of bacterial viability and growth within the biofilm; and (5) the prevention of tooth or apatitic demineralization. In particular, the presently disclosed subject matter provides a composition for the prevention and treatment of an oral disease (e.g., dental caries) that includes one or more iron oxide nanoparticles and hydrogen peroxide. ## Claims ### Claim 1 A method of treating an oral disease comprising: (i) administering to a subject an effective amount of a composition comprising one or more iron nanoparticles; and (ii) administering to the subject an effective amount of hydrogen peroxide. ### Claim 2 (depends on 1) The method of claim 1, wherein the composition further comprises a compound selected from the group consisting of fluoride, copper, calcium phosphate, sodium percarbonate and combinations thereof. ### Claim 3 A method of treating an oral disease comprising administering to a subject an effective amount of a composition comprising one or more iron nanoparticles, wherein the one or more iron nanoparticles comprise nanoparticles conjugated to one or more enzymes selected from the group consisting of matrix degrading enzymes, peroxide producing enzymes and combinations thereof. ### Claim 4 A method of treating a biofilm-associated disease comprising administering to a subject an effective amount of a composition comprising one or more iron nanoparticles, wherein the one or more iron nanoparticles comprise nanoparticles that have a polymeric coating. ### Claim 5 (depends on 1) The method of claim 1, wherein the hydrogen peroxide is administered in a solution that comprises hydrogen peroxide at a concentration from about 0.1% to about 3.0%. ### Claim 6 A method of treating an oral disease comprising administering to a subject an effective amount of a composition comprising one or more iron nanoparticles, wherein the concentration of the iron nanoparticles within the composition is from about 0.01 to about 10.0 mg/ml. ### Claim 7 A method for the elimination and/or treatment of an oral disease comprising: (i) contacting a surface having a biofilm with an effective amount of a composition comprising one or more iron nanoparticles; and (ii) contacting the surface with a solution comprising hydrogen peroxide, wherein the one or more iron nanoparticles and hydrogen peroxide react to form one or more radicals that eliminates and/or treats the oral disease. ### Claim 8 (depends on 1) The method of claim 1, wherein the one or more iron nanoparticles comprise nanoparticles that have a polymeric coating. ### Claim 9 (depends on 1) The method of claim 1, wherein the concentration of the iron nanoparticles within the composition is from about 0.01 to about 10.0 mg/ml. ### Claim 10 (depends on 1) The method of claim 1, wherein the one or more iron nanoparticles are doped with a metal or a salt thereof. ### Claim 11 (depends on 1) The method of claim 1, wherein the one or more iron nanoparticles comprise nanoparticles conjugated to one or more enzymes selected from the group consisting of matrix degrading enzymes, peroxide producing enzymes and combinations thereof. ### Claim 12 (depends on 1) The method of claim 1, wherein the oral disease comprises dental caries. ### Claim 13 (depends on 7) The method of claim 7, wherein the hydrogen peroxide is administered in a solution that comprises hydrogen peroxide at a concentration from about 0.1% to about 3.0%. ### Claim 14 (depends on 7) The method of claim 7, wherein the one or more iron nanoparticles comprise nanoparticles that have a polymeric coating. ### Claim 15 (depends on 7) The method of claim 7, wherein the concentration of the iron nanoparticles within the composition is from about 0.01 to about 10.0 mg/ml. ### Claim 16 (depends on 7) The method of claim 7, wherein the one or more iron nanoparticles are doped with a metal or a salt thereof. ### Claim 17 (depends on 7) The method of claim 7, wherein the one or more iron nanoparticles comprise nanoparticles conjugated to one or more enzymes selected from the group consisting of matrix degrading enzymes, peroxide producing enzymes and combinations thereof. ### Claim 18 A method of treating a biofilm-associated disease comprising: (i) administering to a subject an effective amount of a composition comprising one or more iron nanoparticles; and (ii) administering hydrogen peroxide to the subject at a concentration from about 0.1% to about 3.0%. ### Claim 19 (depends on 18) The method of claim 18, wherein the hydrogen peroxide is present in the composition comprising the one or more iron nanoparticles. ### Claim 20 (depends on 18) The method of claim 18, wherein the composition further comprises a compound selected from the group consisting of fluoride, copper, calcium phosphate, sodium percarbonate and combinations thereof.