# US 10,368,508 B2 · Tomato plants with improved disease resistance

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- Number: US 10,368,508 B2
- Document ID: US10368508B2
- Status: Granted
- Kind: B2
- Application number: 14/882,384
- Filed: 2015-10-13
- Published: 2016-05-12
- Granted: 2019-08-06
- Earliest claimed: 2014-10-15
- Assignees: Seminis Vegetable Seeds, Inc.
- Applicants: Seminis Vegetable Seeds, Inc.
- Inventors: Bart Willem Brugmans; Derek R. Drost; Albert Grit; Jacobus Hoogstraten; Maria Fernanda Rodriguez
- Web page: https://patentgrep.com/patent/US10368508B2

## CPC

- A01H 6/825: {Solanum lycopersicum [tomato]}
- A01H 5/08: Fruits
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The present disclosure provides tomato plants exhibiting resistance to tomato yellow leaf curl virus (TYLCV) and lacking unfavorable linked traits such as necrosis. Such plants may comprise novel introgressed genomic regions associated with disease resistance from S. chilense. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a disease resistance phenotype are provided.

## Claims

### Claim 1

A tomato plant of a cultivated tomato plant variety comprising a recombinant introgression from Solanum chilense on chromosome 6, wherein said recombinant introgression comprises Solanum chilense DNA at locus NSLYC008383071 (SEQ ID NO:1) conferring improved resistance to tomato yellow leaf curl virus relative to a plant lacking said Solanum chilense DNA at locus NSLYC008383071, and wherein said recombinant introgression lacks Solanum chilense DNA at locus NSLYC009077970 (SEQ ID NO:2) genetically linked to said Solanum chilense DNA at locus NSLYC008383071 that confers necrosis when present.

### Claim 2 (depends on 1)

A plant part of the plant of claim 1.

### Claim 3 (depends on 2)

The plant part of claim 2, wherein the plant part is a cell, a seed, a root, a stem, a leaf, a fruit, a flower, or pollen.

### Claim 4

A tomato plant exhibiting improved resistance to tomato yellow leaf curl virus produced by a method comprising the steps of:
(a) obtaining a tomato plant heterozygous for Solanum chilense DNA at locus NSLYC008383071 (SEQ ID NO:1) that confers resistance to tomato yellow leaf curl virus and that is genetically linked in the plant to Solanum chilense DNA at locus NSLYC009077970 (SEQ ID NO:2) that confers necrosis;
(b) obtaining progeny of the plant; and
(c) selecting at least a first progeny plant in which recombination has occurred such that the progeny comprises said Solanum chilense DNA at locus NSLYC008383071 (SEQ ID NO:1) that confers resistance to tomato yellow leaf curl virus but not said Solanum chilense DNA at locus NSLYC009077970 (SEQ ID NO:2) that confers necrosis;
wherein selecting said first progeny plant comprises detecting Solanum chilense DNA at a locus selected from the group consisting of NSLYC009418370 (SEQ ID NO:4), NSLYC008383071 (SEQ ID NO:1), and NL231193.

### Claim 5 (depends on 4)

A part of the plant of claim 4, selected from the group consisting of a cell, a seed, a root, a stem, a leaf, a fruit, a flower, and pollen.