Expression of chimeric KSAC protein and method of producing soluble proteins by high pressure
AssigneeBoehringer Ingelheim Animal Heatlh USA Inc.
InventorsLaurent Bernard Fischer, Nicolas Pierre Yves Carboulec, Fabien Lux
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Abstract
The present invention encompasses vaccines or compositions comprising the chimeric KSAC protein that possesses immunogenic and protective properties, and methods of use including administering to an animal the antigenic KSAC protein thereof to protect animals. The invention also encompasses methods for making and producing the soluble, disaggregated, refolded or active proteins from inclusion bodies produced from prokaryotes or eukaryotes.
Claims10
A composition comprising (i) a refolded and solubilized fusion protein and (ii) dithiothreitol (DTT), wherein the fusion protein consists of leishmania antigens Kinetoplastid Membrane Protein 11 (KMP11), Sterol MethylTransferase (SMT), A2 and Cysteine Proteinase (CP), and wherein the fusion protein comprises the sequence as set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.