Method for propagating microorganisms within plant bioreactors and stably storing microorganisms within agricultural seeds

A method of producing an ACC-deaminase within a bacterial colonized cereal plant bioreactor, the method comprising:

• a. germinating a cereal seed into which at least one inoculant bacterial endophyte has been introduced, wherein the bacterial endophyte is from the genus Burkholderia, comprises a 16S rRNA nucleic acid sequence comprising SEQ ID NO:
• 1446 and can grow on nitrogen free media, to produce the bacterial colonized cereal plant bioreactor;
• and
• b. growing the bacterial colonized cereal plant bioreactor under conditions such that the bacterial endophyte proliferates and the ACC-deaminase is produced in the bacterial colonized cereal plant bioreactor, wherein the ACC-deaminase is produced at a higher concentration in the bacterial colonized cereal plant bioreactor compared to an isoline plant grown from a non-inoculated cereal seed.

A method of producing a ribonucleoside diphosphate reductase within a bacterial colonized cereal plant bioreactor, the method comprising:

• a. germinating a cereal seed into which at least one inoculant bacterial endophyte has been introduced, wherein the bacterial endophyte is from the genus Burkholderia, comprises a 16S rRNA nucleic acid sequence comprising SEQ ID NO:
• 1446 and can grow on nitrogen free media, to produce the bacterial colonized cereal plant bioreactor;
• and
• b. growing the bacterial colonized cereal plant bioreactor under conditions such that the bacterial endophyte proliferates and the ribonucleoside diphosphate reductase is produced in the bacterial colonized cereal plant bioreactor, wherein the ribonucleoside diphosphate reductase is produced at a higher concentration in the bacterial colonized cereal plant bioreactor compared to an isoline plant grown from a non-inoculated cereal seed.