Method of production of 2,4-dihydroxybutyric acid
AssigneeAdisseo France S.A.S.
InventorsThomas Walther, Helene Cordier, Christopher Topham, Isabelle Andre, Magali Remaud-Simeon, Robert Huet, Jean-Marie Francois
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Abstract
A method of producing 2,4-dihydroxybutyric acid (2,4-DHB) by a synthetic pathway that includes transforming malate into 4-phospho-malate using a malate kinase, then transforming 4-phospho-malate into malate-4-semialdehyde using a malate semialdehyde dehydrogenase, and then transforming malate-4-semialdehyde into 2,4-DHB using a DHB dehydrogenase.
Claims41
A malate kinase that transforms malate into 4-phospho-malate;
- the sequence of which is selected from the group consisting of SEQ ID NO:
- 39, 41, 43, and 45.
A malate semialdehyde dehydrogenase that transforms 4-phospho-malate into malate-4-semialdehyde;
- the sequence of which is selected from the group consisting of SEQ ID NO:
- 54, 56, 58, 60, 62, 64, 66, and 231.
A DHB dehydrogenase that transforms malate-4-semialdehyde into 2,4-DHB;
- wherein the DHB dehydrogenase is set forth in SEQ ID NO:
- 81.
Dependencies are inferred from the claim text.