Bacterial cytochrome P450 protein variant and method of reducing concentration of fluorinated methane in sample using the same
AssigneeSamsung Electronics Co., Ltd.
InventorsYukyung Jung, Dongsik Yang, Jinhwan Park, Taeyong Kim, Changduk Kang, Anirban Bhaduri, Tadi Venkata Siva Kumar
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Abstract
Provided is a recombinant microorganism including an exogenous gene encoding a bacterial cytochrome P450 protein or a variant thereof, a composition including the recombinant P450 protein or the variant thereof, which is used for removing CHnF4-n (n is an integer of 0 to 3) in a sample, and a method of reducing a concentration of CHnF4-n in the sample.
Claims31
A bacterial P450Cam variant polypeptide having an amino acid alteration at an amino acid residue corresponding to position F351 of SEQ ID NO:
- 6 selected from the group consisting of F351T, F351N, F351Q, and F351D and having an activity belonging to EC 1.14.15.1.
A method of reducing a concentration of fluorinated methane in a sample, the method comprising contacting a recombinant P450 protein, or a P450Cam variant polypeptide having an amino acid alteration at an amino acid residue corresponding to position F351 of SEQ ID NO:
- 6 and having an activity belonging to EC 1.14.15.1, with the sample comprising fluorinated methane represented by CHnF4-n (n is an integer of 0 to 3) to reduce the concentration of fluorinated methane in the sample.
A method of reducing a concentration of fluorinated methane in a sample, the method comprising contacting a recombinant microorganism with the sample comprising fluorinated methane represented by CHnF4-n (n is an integer of 0 to 3) to reduce the concentration of fluorinated methane in the sample, wherein the recombinant microorganism comprises an exogenous gene encoding a bacterial P450 protein or a variant thereof, wherein the variant is a P450Cam variant having an amino acid alteration at an amino acid residue corresponding to position F351 of SEQ ID NO:
- 6 and having an activity belonging to EC 1.14.15.1.
A bacterial P450BM3 variant polypeptide having an amino acid alteration at an amino acid residue corresponding to position N320 of SEQ ID NO:
- 8 and having an activity belonging to EC 1.14.14.1.
Dependencies are inferred from the claim text.